Combined mass spectrometry-guided genome mining and virtual screening for acaricidal activity in secondary metabolites of Bacillus velezensis W1

doi:10.1039/d1ra01326b

KIB OpenIR

	Combined mass spectrometry-guided genome mining and virtual screening for acaricidal activity in secondary metabolites of Bacillus velezensis W1
	Li,Xingyu ; Munir,Shahzad ; Xu,Yan ; Wang,Yuehu; He,Yueqiu
	2021
发表期刊	RSC ADVANCES
ISSN	2046-2069
卷号	11 期号:41 页码:25441-25449
摘要	A comprehensive analytic strategy was performed to study the acaricidal activity ingredients of Bacillus velezensis W1, a strain for biological control of Tetranychus urticae. Through genome mining, 14 biosynthetic gene clusters were identified, which encode secondary metabolites, and these were further confirmed by MALDI-TOF-MS or LC-ESI-MS/MS, including bacillomycin D C13-C17, macrolactin A, 7-O-malonyl-macrolactin A, surfactin C14, and surfactin C15. Moreover, 27 volatile compounds were identified by GC-MS, mainly including cyclodipeptides, alkanes, organic acids, and esters. Finally, 43 compounds identified from W1 were used in the virtual screening of acaricidal activity. The results showed that 16 compounds, including cyclodipeptides, bacillomycins, macrolactins, and surfactins, have acaricidal potential. This work provides a base for studying the mechanism of acaricidal action of B. velezensis W1 and a comprehensive strategy for the study of active ingredients from biocontrol strains.
关键词	BACILLOMYCIN
DOI	10.1039/d1ra01326b
WOS记录号	WOS:000678294800029
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73497
专题	中国科学院昆明植物研究所
作者单位	1.Yunnan Agr Univ, Coll Sci, Kunming 650201, Yunnan, Peoples R China 2.Cleveland State Univ, Dept Chem, Cleveland, OH 44115 USA 3.Yunnan Agr Univ, State Key Lab Conservat & Utilizat Bioresources, Kunming 650201, Yunnan, Peoples R China 4.Chinese Acad Sci, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Li,Xingyu,Munir,Shahzad,Xu,Yan,et al. Combined mass spectrometry-guided genome mining and virtual screening for acaricidal activity in secondary metabolites of Bacillus velezensis W1[J]. RSC ADVANCES,2021,11(41):25441-25449.
APA	Li,Xingyu,Munir,Shahzad,Xu,Yan,Wang,Yuehu,&He,Yueqiu.(2021).Combined mass spectrometry-guided genome mining and virtual screening for acaricidal activity in secondary metabolites of Bacillus velezensis W1.RSC ADVANCES,11(41),25441-25449.
MLA	Li,Xingyu,et al."Combined mass spectrometry-guided genome mining and virtual screening for acaricidal activity in secondary metabolites of Bacillus velezensis W1".RSC ADVANCES 11.41(2021):25441-25449.