Paris Saponin VII Induces Apoptosis and Cell Cycle Arrest in Erythroleukemia Cells by a Mitochondrial Membrane Signaling Pathway

doi:10.2174/1871520620666200615134039

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	Paris Saponin VII Induces Apoptosis and Cell Cycle Arrest in Erythroleukemia Cells by a Mitochondrial Membrane Signaling Pathway
	Lin,Xin ; Gajendran,Babu ; Varier,Krishnapriya M.; Liu,Wuling ; Song,Jingrui ; Rao,Qing ; Wang,Chunlin ; Qiu,Jianfei ; Ni,Wei ; Qin,XuJie; Wen,Min ; Liu,Haiyang; Li,Yanmei
	2021
发表期刊	ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
ISSN	1871-5206
卷号	21 期号:4 页码:498-507
摘要	Background and Purpose: Leukemia is considered a top-listed ailment, according to WHO, which contributes to the death of a major population of the world every year. Paris Saponin VII (PS), a saponin which was isolated from the roots of Trillium kamtschaticum, from our group, was reported to provide hemostatic, cytotoxic and antimicrobial activities. However, its molecular mechanism underlying the anti-proliferative effects remains unclear. Thus, this study hypothesized to assess that mechanism in PS treated HEL cells.Methods: The MTT assay was used to analyze the PS inhibited cell viability in the HEL cells. We further found that PS could induce S phase cell cycle arrest through flow cytometry as well as the western blot analysis of intrinsic and extrinsic apoptotic molecules.Results: The MTT assay showed the IC50 concentration of PS as 0.667 mu M. The study revealed that PS treatment inhibits cell proliferation dose-dependently. It further caused mitochondrial membrane potential changes by PS treatment. Mechanistic protein expression revealed a dose-dependent upsurge for Bid and Bim molecules, while Bcl2 and PARP expression levels were significantly (P<0.05) down-regulated in PS treated HEL cells resulting in caspase-3 release and increased the Bim levels upon 24h of incubation.Conclusion: These findings indicate that PS possesses an excellent anti-leukemic activity via the regulation of the mitochondrial pathway, leading to S phase cell cycle arrest and caspase-dependent apoptosis, suggesting it as a potential alternative chemotherapeutic agent for leukemia patients.
关键词	Leukemia Paris Saponin VII HEL cell cycle apoptosis membrane signaling pathway
DOI	10.2174/1871520620666200615134039
WOS记录号	WOS:000631784000002
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73494
专题	中国科学院昆明植物研究所
作者单位	1.Guizhou Med Univ, State Key Lab Funct & Applicat Med Plants, Guiyang 550014, Guizhou, Peoples R China 2.Dept Biol & Chem, Key Lab Chem Nat Prod Guizhou Prov, Guiyang 550014, Guizhou, Peoples R China 3.Chinese Acad Sci, Guiyang 550014, Guizhou, Peoples R China 4.Chengdu Univ, Coll Med, Chengdu, Peoples R China 5.Varier, Krishnapriya M.] Univ Madras, Dr ALM PGIBMS, Dept Med Biochem, Taramani Campus, Madras 600113, Tamil Nadu, India 6.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 7.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Nat Med Chem, Kunming 650201, Yunnan, Peoples R China 8.Guizhou Med Univ, Coll Basic Med, Guiyang, Guizhou, Peoples R China
推荐引用方式 GB/T 7714	Lin,Xin,Gajendran,Babu,Varier,Krishnapriya M.,et al. Paris Saponin VII Induces Apoptosis and Cell Cycle Arrest in Erythroleukemia Cells by a Mitochondrial Membrane Signaling Pathway[J]. ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY,2021,21(4):498-507.
APA	Lin,Xin.,Gajendran,Babu.,Varier,Krishnapriya M..,Liu,Wuling.,Song,Jingrui.,...&Li,Yanmei.(2021).Paris Saponin VII Induces Apoptosis and Cell Cycle Arrest in Erythroleukemia Cells by a Mitochondrial Membrane Signaling Pathway.ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY,21(4),498-507.
MLA	Lin,Xin,et al."Paris Saponin VII Induces Apoptosis and Cell Cycle Arrest in Erythroleukemia Cells by a Mitochondrial Membrane Signaling Pathway".ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY 21.4(2021):498-507.