KIB OpenIR
Unveiling the Disaccharide-Branched Glycosaminoglycan and Anticoagulant Potential of Its Derivatives
Yin,Ronghua; Zhou,Lutan; Gao,Na; Lin,Lisha; Sun,Huifang; Chen,Dingyuan; Cai,Ying; Zuo,Zhili; Hu,Kaifeng; Huang,Shengxiong; Liu,Jikai; Zhao,Jinhua
2021
Source PublicationBIOMACROMOLECULES
ISSN1525-7797
Volume22Issue:3Pages:1244-1255
AbstractGlycosaminoglycans (GAGs) are conserved polysaccharides composed of linear repeating disaccharides and play crucial roles in multiple biological processes in animal kingdom. However, saccharide-branched GAGs are rarely found, except the fucose-branched one from sea cucumbers. There was conjecture about the presence of disaccharide-branched GAG since 30 years ago, though not yet confirmed. Here, we report a GAG containing galactose-fucose branches from Thelenota ananas. This unique branch was confirmed as D-Gal(4)S(6S)-alpha 1,2-L-Fuc(3S) by structural elucidation of oligosaccharides prepared from T. ananas GAG. Bioassays indicated that oligomers with a larger degree of polymerization exhibited a potent anticoagulation by targeting the intrinsic tenase. Heptasaccharide was proven as the minimum fragment retaining the anticoagulant potential and showed 92.6% inhibition of venous thrombosis in vivo at sc. of 8 mg/kg with no obvious bleeding risks. These results not only solve a long-standing question about the presence of disaccharide-branched GAG in Holothuroidea, but open up new opportunities to develop safer anticoagulants.
KeywordFUCOSYLATED CHONDROITIN SULFATE DEPOLYMERIZED HOLOTHURIAN GLYCOSAMINOGLYCAN INTRINSIC TENASE COMPLEX SEA-CUCUMBER FUCOSE BRANCHES BODY-WALL INDEPENDENT INHIBITION POLYSACCHARIDE ACCOUNT FACTOR XASE HEPARIN
DOI10.1021/acs.biomac.0c01739
WOS IDWOS:000627592800019
Citation statistics
Cited Times:11[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/73466
Collection中国科学院昆明植物研究所
Affiliation1.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan 430074, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Yin,Ronghua,Zhou,Lutan,Gao,Na,et al. Unveiling the Disaccharide-Branched Glycosaminoglycan and Anticoagulant Potential of Its Derivatives[J]. BIOMACROMOLECULES,2021,22(3):1244-1255.
APA Yin,Ronghua.,Zhou,Lutan.,Gao,Na.,Lin,Lisha.,Sun,Huifang.,...&Zhao,Jinhua.(2021).Unveiling the Disaccharide-Branched Glycosaminoglycan and Anticoagulant Potential of Its Derivatives.BIOMACROMOLECULES,22(3),1244-1255.
MLA Yin,Ronghua,et al."Unveiling the Disaccharide-Branched Glycosaminoglycan and Anticoagulant Potential of Its Derivatives".BIOMACROMOLECULES 22.3(2021):1244-1255.
Files in This Item: Download All
File Name/Size DocType Version Access License
111-H10-219-尹荣华-Biom(4401KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Yin,Ronghua]'s Articles
[Zhou,Lutan]'s Articles
[Gao,Na]'s Articles
Baidu academic
Similar articles in Baidu academic
[Yin,Ronghua]'s Articles
[Zhou,Lutan]'s Articles
[Gao,Na]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Yin,Ronghua]'s Articles
[Zhou,Lutan]'s Articles
[Gao,Na]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 111-H10-219-尹荣华-Biomacromolecule.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.