Prostaglandin E1 Is an Efficient Molecular Tool for Forest Leech Blood Sucking

doi:10.3389/fvets.2020.615915

KIB OpenIR

	Prostaglandin E1 Is an Efficient Molecular Tool for Forest Leech Blood Sucking
	Zheng,Fenshuang ; Zhang,Min ; Yang,Xingwei; Wu,Feilong ; Wang,Gan ; Feng,Xingxing ; Ombati,Rose ; Zuo,Ruiling ; Yang,Canju ; Liu,Jun ; Lai,Ren ; Luo,Xiaodong; Long,Chengbo
	2021
发表期刊	FRONTIERS IN VETERINARY SCIENCE
ISSN	2297-1769
卷号	7 页码:615915
摘要	From a survival perspective, it is hypothesized that leech saliva exhibits certain physiological effects to ensure fast blood-feeding, including analgesia, anesthesia, and anti-inflammation to stay undetected by the host and vasodilatation and anti-hemostasis to ensure a steady, rapid, and sustained blood flow to the feeding site. Many anti-hemostatic compounds have been identified in leech saliva, such as hirudin, calin, and bdellin A. However, no specific substance with direct vasodilatory and anti-inflammatory function has been reported from forest leech saliva. Herein, using activity-guided analysis, prostaglandin E1 (PGE1) was identified for the first time as an efficient molecular tool for forest leech blood sucking. The structure of PGE1 was analyzed by nuclear magnetic resonance spectroscopy and high-resolution electrospray ionization mass spectroscopy. PGE1 was found to be primarily distributed in the leech salivary gland (1228.36 ng/g body weight). We also analyzed how forest leech PGE1 affects platelet aggregation, skin vascular permeability, bleeding time, and pain. Results indicated that PGE1 efficiently inhibited platelet aggregation induced by adenosine diphosphate (ADP) (5 mu M) with an IC50 of 21.81 +/- 2.24 nM. At doses of 10, 100 nM, and 1 mu M, PGE1 increased vascular permeability by 1.18, 5.8, and 9.2 times. It also prolonged bleeding time in a concentration-independent manner. In the formalin-induced mouse paw pain model, PGE1 suppressed acute pain. To the best of our knowledge, this is the first report on PGE1 in invertebrates. The functions of PGE1, such as vasodilation, platelet aggregation inhibition, anti-inflammation, and pain alleviation, may facilitate the ingestion of host blood by leeches.
关键词	leech blood-sucking prostaglandin E1 anticoagulant Haemadipsa sylvestris MEDICINAL LEECH PGE1 INFLAMMATION THERAPY PAIN E-1
DOI	10.3389/fvets.2020.615915
WOS记录号	WOS:000609134500001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73446
专题	中国科学院昆明植物研究所
作者单位	1.Second Peoples Hosp Yunnan Prov, Dept Emergency Med, Kunming, Yunnan, Peoples R China 2.Chinese Acad Sci & Yunnan Prov, Key Lab Anim Models & Human Dis Mech, Kunming Inst Zool, Kunming, Yunnan, Peoples R China 3.Univ Chinese Acad Sci, Kunming Coll Life Sci, Kunming, Yunnan, Peoples R China 4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Yunnan, Peoples R China 5.Kunming Childrens Hosp, Dept Clin Lab, Kunming, Yunnan, Peoples R China 6.Dali Peoples Hosp Yunnan Prov, Dali, Peoples R China 7.Dehong Peoples Hosp, Mangshi, Peoples R China 8.Chinese Acad Sci, SinoAfrican Joint Res Ctr, Wuhan, Peoples R China
推荐引用方式 GB/T 7714	Zheng,Fenshuang,Zhang,Min,Yang,Xingwei,et al. Prostaglandin E1 Is an Efficient Molecular Tool for Forest Leech Blood Sucking[J]. FRONTIERS IN VETERINARY SCIENCE,2021,7:615915.
APA	Zheng,Fenshuang.,Zhang,Min.,Yang,Xingwei.,Wu,Feilong.,Wang,Gan.,...&Long,Chengbo.(2021).Prostaglandin E1 Is an Efficient Molecular Tool for Forest Leech Blood Sucking.FRONTIERS IN VETERINARY SCIENCE,7,615915.
MLA	Zheng,Fenshuang,et al."Prostaglandin E1 Is an Efficient Molecular Tool for Forest Leech Blood Sucking".FRONTIERS IN VETERINARY SCIENCE 7(2021):615915.