Bioactivity Ingredients of Chaenomeles speciosa against Microbes: Characterization by LC-MS and Activity Evaluation

doi:10.1021/acs.jafc.1c00298

KIB OpenIR

	Bioactivity Ingredients of Chaenomeles speciosa against Microbes: Characterization by LC-MS and Activity Evaluation
	Wang,Zhao-Jie ; Jin,Dan-Ni ; Zhou,Ying ; Sang,Xu-Yan ; Zhu,Yan-Yan ; He,Ying-Jie ; Xie,Tian-Zhen ; Dai,Zhi ; Zhao,Yun-Li ; Luo,Xiao-Dong
	2021
发表期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN	0021-8561
卷号	69 期号:16 页码:4686-4696
摘要	Chaenomeles speciosa (Sweet) Nakai is a dual-purpose Chinese herbal medicine and functional food favored by minorities in Southwest China, and its fruits are used for the treatment of dyspepsia, dysentery, enteritis, and rheumatism inflammation. Some diseases may be related to microbial infection; however, it is not known how the fruits possess antimicrobial activity. We evaluated the antimicrobial bioctivity of different evaluation extracts of C. speciosa fruits by in vitro and in vivo with colony-forming unit assays, and the strongest bioactive-guided fraction was selected for column chromatography (CC), UHPLC-QTOF-MS/MS, and NMR spectroscopy to confirm the chemical constituents. The most possible antimicrobial mechanism of C. speciosa fruits was explored by metabolomics approach, fluorescence microscopy imaging, and scanning electron microscopy (SEM). Thirty compounds, which were major characteristic ions of the bioactive fraction, were determined precisely. The bioactive fraction could inhibit 18 pathogenic microorganisms, significantly reduced, especially drug-resistant bacteria, compared to ampicillin sodium salt, fluconazole, and berberine chloride form; and the minimum inhibitory concentration (MIC) or minimum fungicidal concentration (MFC) values were in the range of 0.1-1 mg/mL. The compounds 2'-methoxyaucuparin (1) and oleanolic acid (20) not only have antibacterial activity but also may have synergistic effects. Further, the bioactive fraction might inhibit the biofilm formation, enhance immunity, and restore bacterial infection damage in vitro and in vivo to kill microorganisms. The data indicated that C. speciosa fruits' major bioactive fraction enriched with triterpenes, flavonoids, and phenolics could be developed as a functional supplement for individuals to prevent and treat microbial infection.
关键词	antimicrobial Chaenomeles speciosa LC-MS metabolomics LUNG INFLAMMATION ANTIOXIDANT FRUITS POLYSACCHARIDE
DOI	10.1021/acs.jafc.1c00298
WOS记录号	WOS:000645559200012
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73422
专题	中国科学院昆明植物研究所
作者单位	1.[Wang, Zhao-Jie 2.Jin, Dan-Ni 3.Zhou, Ying 4.Sang, Xu-Yan 5.Zhu, Yan-Yan 6.He, Ying-Jie 7.Xie, Tian-Zhen 8.Dai, Zhi 9.Zhao, Yun-Li 10.Yunnan Univ, Minist Educ, Key Lab Med Chem Nat Resource, Sch Chem Sci & Technol, Kunming 650091, Yunnan, Peoples R China 11.Yunnan Univ, Yunnan Prov Ctr Res & Dev Nat Prod, Sch Chem Sci & Technol, Kunming 650091, Yunnan, Peoples R China 12.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Wang,Zhao-Jie,Jin,Dan-Ni,Zhou,Ying,et al. Bioactivity Ingredients of Chaenomeles speciosa against Microbes: Characterization by LC-MS and Activity Evaluation[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2021,69(16):4686-4696.
APA	Wang,Zhao-Jie.,Jin,Dan-Ni.,Zhou,Ying.,Sang,Xu-Yan.,Zhu,Yan-Yan.,...&Luo,Xiao-Dong.(2021).Bioactivity Ingredients of Chaenomeles speciosa against Microbes: Characterization by LC-MS and Activity Evaluation.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,69(16),4686-4696.
MLA	Wang,Zhao-Jie,et al."Bioactivity Ingredients of Chaenomeles speciosa against Microbes: Characterization by LC-MS and Activity Evaluation".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 69.16(2021):4686-4696.