ZmMPK6 and ethylene signalling negatively regulate the accumulation of anti-insect metabolites DIMBOA and DIMBOA-Glc in maize inbred line A188

doi:10.1111/nph.16974

KIB OpenIR

	ZmMPK6 and ethylene signalling negatively regulate the accumulation of anti-insect metabolites DIMBOA and DIMBOA-Glc in maize inbred line A188
	Zhang,Cuiping ; Li,Jing; Li,Sen ; Ma,Canrong ; Liu,Hui ; Wang,Lei; Qi,Jinfeng ; Wu,Jianqiang
	2021
发表期刊	NEW PHYTOLOGIST
ISSN	0028-646X
卷号	229 期号:4 页码:2273-2287
摘要	2,4-Dihydroxy-7-methoxy-1,4-benzoxazin-3-one (DIMBOA) and DIMBOA-glucoside (DIMBOA-Glc) are anti-insect benzoxazinoids in maize, yet very little information is known about how they are regulated.Reverse genetics, kinase activity analysis, phytohormone and DIMBOA/DIMBOA-Glc quantification, bioassays and transcriptome analysis were employed to study the function of ZmMPK6, a mitogen-activated protein kinase, in maize response to herbivory.ZmMPK6 was rapidly activated by wounding and simulated herbivory. Silencing ZmMPK6 in maize A188 compromised simulated herbivory-induced ethylene levels but not those of jasmonic acid or salicylic acid, and the ZmMPK6-silenced plants exhibited elevated DIMBOA/DIMBOA-Glc and insect resistance. An ethylene complementation experiment revealed that ZmMPK6 repressed the accumulation of DIMBOA/DIMBOA-Glc in an ethylene-dependent manner. Transcriptome analysis revealed that ZmMPK6 might meditate the transcription of BX1 by controlling a MYB transcription factor that is likely to be located in the ethylene signalling pathway and, furthermore, ZmMPK6 and ethylene signalling also specifically and commonly regulate the transcription of other benzoxazinoid biosynthetic genes. We also show that different maize lines have very different responses to simulated herbivory in terms of ZmMPK6 activation, ethylene emission and benzoxazinoid levels.These results uncover that ZmMPK6 and ethylene pathway are novel repressors of DIMBOA/DIMBOA-Glc and provide new insight into the regulatory mechanisms underlying these two pathways.
关键词	DIMBOA ethylene herbivory maize MPK6 Mythimna separata transcriptome ACTIVATED PROTEIN-KINASE NICOTIANA-ATTENUATA ORAL SECRETIONS JASMONIC ACID TRANSCRIPTION FACTORS EXPRESSION ANALYSIS DEFENSE RESPONSES APHID RESISTANCE PLANT DEFENSES BIOSYNTHESIS
DOI	10.1111/nph.16974
WOS记录号	WOS:000583521700001
引用统计	被引频次：18[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73359
专题	中国科学院昆明植物研究所
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resources, Dept Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, CAS Ctr Excellence Biot Interact, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Zhang,Cuiping,Li,Jing,Li,Sen,et al. ZmMPK6 and ethylene signalling negatively regulate the accumulation of anti-insect metabolites DIMBOA and DIMBOA-Glc in maize inbred line A188[J]. NEW PHYTOLOGIST,2021,229(4):2273-2287.
APA	Zhang,Cuiping.,Li,Jing.,Li,Sen.,Ma,Canrong.,Liu,Hui.,...&Wu,Jianqiang.(2021).ZmMPK6 and ethylene signalling negatively regulate the accumulation of anti-insect metabolites DIMBOA and DIMBOA-Glc in maize inbred line A188.NEW PHYTOLOGIST,229(4),2273-2287.
MLA	Zhang,Cuiping,et al."ZmMPK6 and ethylene signalling negatively regulate the accumulation of anti-insect metabolites DIMBOA and DIMBOA-Glc in maize inbred line A188".NEW PHYTOLOGIST 229.4(2021):2273-2287.