Insights into triterpene synthesis and unsaturated fatty-acid accumulation provided by chromosomal-level genome analysis of Akebia trifoliata subsp. australis

doi:10.1038/s41438-020-00458-y

KIB OpenIR

	Insights into triterpene synthesis and unsaturated fatty-acid accumulation provided by chromosomal-level genome analysis of Akebia trifoliata subsp. australis
	Huang,Hui ; Liang,Juan ; Tan,Qi ; Ou,Linfeng ; Li,Xiaolin ; Zhong,Caihong ; Huang,Huilin ; Moller,Ian Max ; Wu,Xianjin ; Song,Songquan
	2021
发表期刊	HORTICULTURE RESEARCH
ISSN	2662-6810
卷号	8 期号:1 页码:33
摘要	Akebia trifoliata subsp. australis is a well-known medicinal and potential woody oil plant in China. The limited genetic information available for A. trifoliata subsp. australis has hindered its exploitation. Here, a high-quality chromosome-level genome sequence of A. trifoliata subsp. australis is reported. The de novo genome assembly of 682.14Mb was generated with a scaffold N50 of 43.11Mb. The genome includes 25,598 protein-coding genes, and 71.18% (485.55Mb) of the assembled sequences were identified as repetitive sequences. An ongoing massive burst of long terminal repeat (LTR) insertions, which occurred similar to 1.0 million years ago, has contributed a large proportion of LTRs in the genome of A. trifoliata subsp. australis. Phylogenetic analysis shows that A. trifoliata subsp. australis is closely related to Aquilegia coerulea and forms a clade with Papaver somniferum and Nelumbo nucifera, which supports the well-established hypothesis of a close relationship between basal eudicot species. The expansion of UDP-glucoronosyl and UDP-glucosyl transferase gene families and beta-amyrin synthase-like genes and the exclusive contraction of terpene synthase gene families may be responsible for the abundant oleanane-type triterpenoids in A. trifoliata subsp. australis. Furthermore, the acyl-ACP desaturase gene family, including 12 stearoyl-acyl-carrier protein desaturase (SAD) genes, has expanded exclusively. A combined transcriptome and fatty-acid analysis of seeds at five developmental stages revealed that homologs of SADs, acyl-lipid desaturase omega fatty acid desaturases (FADs), and oleosins were highly expressed, consistent with the rapid increase in the content of fatty acids, especially unsaturated fatty acids. The genomic sequences of A. trifoliata subsp. australis will be a valuable resource for comparative genomic analyses and molecular breeding.
关键词	READ ALIGNMENT OIL BODIES GENES IDENTIFICATION ANNOTATION RETROTRANSPOSONS CAROTENOIDS OLEOSINS SAPONINS DATABASE
DOI	10.1038/s41438-020-00458-y
WOS记录号	WOS:000616721100015
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73311
专题	中国科学院昆明植物研究所
作者单位	1.Huaihua Univ, Coll Biol & Food Engn, Key Lab Res & Utilizat Ethnomed Plant Resources H, Huaihua 418000, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China 3.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Breeding Base Dao di Herbs, Beijing 100700, Peoples R China 4.Aarhus Univ, Dept Mol Biol & Genet, DK-4200 Slagelse, Denmark 5.Chinese Acad Sci, Inst Bot, Beijing 100093, Peoples R China
推荐引用方式 GB/T 7714	Huang,Hui,Liang,Juan,Tan,Qi,et al. Insights into triterpene synthesis and unsaturated fatty-acid accumulation provided by chromosomal-level genome analysis of Akebia trifoliata subsp. australis[J]. HORTICULTURE RESEARCH,2021,8(1):33.
APA	Huang,Hui.,Liang,Juan.,Tan,Qi.,Ou,Linfeng.,Li,Xiaolin.,...&Song,Songquan.(2021).Insights into triterpene synthesis and unsaturated fatty-acid accumulation provided by chromosomal-level genome analysis of Akebia trifoliata subsp. australis.HORTICULTURE RESEARCH,8(1),33.
MLA	Huang,Hui,et al."Insights into triterpene synthesis and unsaturated fatty-acid accumulation provided by chromosomal-level genome analysis of Akebia trifoliata subsp. australis".HORTICULTURE RESEARCH 8.1(2021):33.