Impact of titanium dioxide nanoparticles on intestinal community in 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute colitis mice and the intervention effect of vitamin E

doi:10.1039/d0nr08106j

KIB OpenIR

	Impact of titanium dioxide nanoparticles on intestinal community in 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute colitis mice and the intervention effect of vitamin E
	Gao,Yanjun ; Li,Tingyu ; Duan,Shumin ; Lyu,Lizhi ; Li,Yuan ; Xu,Lin ; Wang,Yun
	2021
发表期刊	NANOSCALE
ISSN	2040-3364
卷号	13 期号:3 页码:1842-1862
摘要	Titanium dioxide nanoparticles (TiO2-NPs) are widely applied as additives in foods due to their excellent whitening and brightening capability. Although the toxicity and antibacterial activity of TiO2-NPs have been extensively studied, their impact on the gut microbiota in vivo still remains unclear, especially in animals with gastrointestinal disorders. In the present study, healthy mice and TNBS-induced colitis mice were administered with TiO2-NPs (38.3 +/- 9.3 nm) orally at a dose of 100 mg per kg bw daily for 10 days to study the impact of TiO2-NPs on the gut microbiota and colitis development. Moreover, the mechanism of TiO2-NPs on the gut microbiota was also discussed when the colitis mice were additionally administered with vitamin E to remove ROS. Changes in the microbiota community structure and gut-associated function prediction were analyzed through bioinformatics. The result showed that the oral administration of TiO2-NPs mitigated colitis symptoms by reducing the DAI and CMDI scores and TNF-alpha level. Furthermore, 16S rDNA sequencing analysis showed that the structure and function prediction of gut microbiota could be modified in healthy mice and colitis mice after exposure to TiO2-NPs, but the opposite physiological effect occurred since the dominant flora varied in these two groups. Moreover, vitamin E intervention did not change the effects of TiO2-NPs on the microbiota community structure and gut-associated function, which indicates that the mechanism of the biological effects of TiO2-NPs on the gut microbiota may not be associated with their ability to induce the generation of ROS. In summary, our work firstly found that TiO2-NPs could regulate the gut microbiota of colitis mice and participate in the mitigation of TNBS-induced acute colitis, and the capability of TiO2-NPs to induce the generation of ROS inducement did not contribute to this process.
关键词	GUT MICROBIOTA GLUTAMINE-SYNTHETASE ULCERATIVE-COLITIS INFLAMMATION METABOLISM FOOD ANTIOXIDANT TRYPTOPHAN BUTYRATE PROTECTS
DOI	10.1039/d0nr08106j
WOS记录号	WOS:000612999200038
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73242
专题	中国科学院昆明植物研究所
作者单位	1.Peking Univ, Sch Publ Hlth, Dept Occupat & Environm Hlth Sci, Beijing 100191, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China 3.Northwest Univ, Coll Life Sci, 1 Taibai North Rd, Xian 710069, Peoples R China
推荐引用方式 GB/T 7714	Gao,Yanjun,Li,Tingyu,Duan,Shumin,et al. Impact of titanium dioxide nanoparticles on intestinal community in 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute colitis mice and the intervention effect of vitamin E[J]. NANOSCALE,2021,13(3):1842-1862.
APA	Gao,Yanjun.,Li,Tingyu.,Duan,Shumin.,Lyu,Lizhi.,Li,Yuan.,...&Wang,Yun.(2021).Impact of titanium dioxide nanoparticles on intestinal community in 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute colitis mice and the intervention effect of vitamin E.NANOSCALE,13(3),1842-1862.
MLA	Gao,Yanjun,et al."Impact of titanium dioxide nanoparticles on intestinal community in 2,4,6-trinitrobenzenesulfonic acid (TNBS)-induced acute colitis mice and the intervention effect of vitamin E".NANOSCALE 13.3(2021):1842-1862.