Identification of Bioactive Phytochemicals from Six Plants: Mechanistic Insights into the Inhibition of Rumen Protozoa, Ammoniagenesis, and alpha-Glucosidase

doi:10.3390/biology10101055

KIB OpenIR

	Identification of Bioactive Phytochemicals from Six Plants: Mechanistic Insights into the Inhibition of Rumen Protozoa, Ammoniagenesis, and alpha-Glucosidase
	Ayemele,Aurele Gnetegha ; Ma,Lu ; Li,Xiumei ; Yang,Peilong ; Xu,Jianchu; Yu,Zhongtang ; Bu,Dengpan
	2021
发表期刊	BIOLOGY-BASEL
ISSN	2079-7737
卷号	10 期号:10 页码:1055
摘要	Simple Summary:& nbsp;Rumen protozoa have some contribution to feed digestibility in the rumen, but Entodinium, the most predominant genus, is the main culprit of inefficient nitrogen utilization in ruminants. Using chemical drugs, many studies have attempted to inhibit the rumen protozoa, but few of the approaches are either effective or practical. In this study, we investigated the nutritional and functional properties of Adansonia digitata (baobab), Flemingia macrophylla (waras tree), Kalimeris indica (Indian aster), Brassica rapa subsp. chinensis (bok choy), Portulaca oleracea (common purslane), and Calotropis gigantea (giant milkweed) for their potential as feed additives in animal husbandry. The plants were also analyzed for their major phytochemicals using reversed phase-high performance liquid chromatography (HPLC) and then evaluated for their ability to inhibit rumen protozoa, ammoniagenesis, and microbial a-glucosidase activity in vitro. C. gigantea inhibited the rumen protozoa and reduced the wasteful ammoniagenesis, thereby indicating improved nitrogen utilization. A. digitata also reduced the microbial a-glucosidase activity that can potentially contribute to rumen acidosis. The tested plants, especially C. gigantea and A. digitata, could be used as potential alternatives to chemicals or antibiotics to ensure sustainable and green animal husbandry. Rumen protozoa prey on feed-degrading bacteria synthesizing microbial protein, lowering nitrogen utilization efficiency in ruminants. In this in vitro study, we evaluated six plants (Adansonia digitata, Flemingia macrophylla, Kalimeris indica, Brassica rapa subsp. chinensis, Portulaca oleracea, and Calotropis gigantea) for their potential to inhibit rumen protozoa and identified the phytochemicals potentially responsible for protozoa inhibition. Rumen protozoa were anaerobically cultured in vitro in the presence of each plant at four doses. All of the tested plants reduced total rumen protozoa (p & LE; 0.05), but C. gigantea and B. rapa were the most inhibitory, inhibiting rumen protozoa by 45.6 and 65.7%, respectively, at the dose of 1.1 mg/mL. Scanning electron microscopy revealed a disruption of the extracellular structure of protozoa cells. Only C. gigantea also decreased the wasteful ammoniagenesis (p & LE; 0.05). Moreover, the A. digitata extract inhibited a-glucosidase activity by about 70% at 100 mu g/mL. Reversed-phase high-performance liquid chromatography analysis detected quercetin, anthraquinone, 3-hydroxybenzoic acid, astragaloside, and myricetin in the tested plant leaves. These plants may hold potential as feed additives to reduce rumen protozoa and a- glucosidase activity. Future research is needed to identify the specific anti-protozoal compound(s), the effects on the rumen microbiome, and its fermentation characteristics.
关键词	bioactive phytochemicals feed efficiency microbial alpha-glucosidase protozoa inhibition SUBACUTE RUMINAL ACIDOSIS ENTODINIUM-CAUDATUM L. LEAVES FERMENTATION PERFORMANCE GLYCOSIDES ACARBOSE GROWTH
DOI	10.3390/biology10101055
WOS记录号	WOS:000714021300001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73179
专题	中国科学院昆明植物研究所
作者单位	1.Chinese Acad Agr Sci, Inst Anim Sci, State Key Lab Anim Nutr, Beijing 100193, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Honghe Ctr Mt Futures, Kunming 654400, Yunnan, Peoples R China 3.Chinese Acad Agr Sci, Feed Res Inst, Beijing 100081, Peoples R China 4.World Agroforestry Ctr, Kunming 650201, Yunnan, Peoples R China 5.Ohio State Univ, Dept Anim Sci, Columbus, OH 43210 USA 6.Chinese Acad Agr Sci CAAS, Joint Lab Integrated Crop Tree Livestock Syst, Ethiopian Inst Agr Res EIAR, Beijing 100193, Peoples R China 7.World Agroforestry Ctr ICRAF, Beijing 100193, Peoples R China
推荐引用方式 GB/T 7714	Ayemele,Aurele Gnetegha,Ma,Lu,Li,Xiumei,et al. Identification of Bioactive Phytochemicals from Six Plants: Mechanistic Insights into the Inhibition of Rumen Protozoa, Ammoniagenesis, and alpha-Glucosidase[J]. BIOLOGY-BASEL,2021,10(10):1055.
APA	Ayemele,Aurele Gnetegha.,Ma,Lu.,Li,Xiumei.,Yang,Peilong.,Xu,Jianchu.,...&Bu,Dengpan.(2021).Identification of Bioactive Phytochemicals from Six Plants: Mechanistic Insights into the Inhibition of Rumen Protozoa, Ammoniagenesis, and alpha-Glucosidase.BIOLOGY-BASEL,10(10),1055.
MLA	Ayemele,Aurele Gnetegha,et al."Identification of Bioactive Phytochemicals from Six Plants: Mechanistic Insights into the Inhibition of Rumen Protozoa, Ammoniagenesis, and alpha-Glucosidase".BIOLOGY-BASEL 10.10(2021):1055.