A 2-oxoglutarate-dependent dioxygenase converts dihydrofuran to furan in Salvia diterpenoids

doi:10.1093/plphys/kiab567

KIB OpenIR

	A 2-oxoglutarate-dependent dioxygenase converts dihydrofuran to furan in Salvia diterpenoids
	Song,Jiao-Jiao ; Fang,Xin ; Li,Chen-Yi ; Jiang,Yan ; Li,Jian-Xu ; Wu,Sheng ; Guo,Juan ; Liu,Yan ; Fan,Hang ; Huang,Yan-Bo ; Wei,Yu-Kun ; Kong,Yu ; Zhao,Qing ; Xu,Jing-Jing ; Hu,Yong-Hong ; Chen,Xiao-Ya ; Yang,Lei
	2022
发表期刊	PLANT PHYSIOLOGY
ISSN	0032-0889
卷号	188 期号:3 页码:1496-1506
摘要	A 2-oxoglutarate-dependent dioxygenase is responsible for tanshinone IIA biosynthesis in medicinal herb Salvia miltiorrhiza, which controls the metabolite flux from dihydrofuran- to furan-tanshinones.Tanshinone IIA (TIIA), a diterpene quinone with a furan ring, is a bioactive compound found in the medicinal herb redroot sage (Salvia miltiorrhiza Bunge), in which both furan and dihydrofuran analogs are present in abundance. Progress has been made recently in elucidating the tanshinone biosynthetic pathway, including heterocyclization of the dihydrofuran D-ring by cytochrome P450s; however, dehydrogenation of dihydrofuran to furan, a key step of furan ring formation, remains uncharacterized. Here, by differential transcriptome mining, we identified six 2-oxoglutarate-dependent dioxygenase (2-ODD) genes whose expressions corresponded to tanshinone biosynthesis. We showed that Sm2-ODD14 acts as a dehydrogenase catalyzing the furan ring aromatization. In vitro Sm2-ODD14 converted cryptotanshinone to TIIA and thus was designated TIIA synthase (SmTIIAS). Furthermore, SmTIIAS showed a strict substrate specificity, and repression of SmTIIAS expression in hairy root by RNAi led to increased accumulation of total dihydrofuran-tanshinones and decreased production of furan-tanshinones. We conclude that SmTIIAS controls the metabolite flux from dihydrofuran- to furan-tanshinones, which influences medicinal properties of S. miltiorrhiza.
关键词	MOLECULAR-CLONING BIOSYNTHESIS TANSHINONES EVOLUTION CYTOTOXICITY SUPERFAMILY OXYGENASES APOPTOSIS SYNTHASE
DOI	10.1093/plphys/kiab567
WOS记录号	WOS:000764825700001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73159
专题	中国科学院昆明植物研究所
作者单位	1.Shanghai Chenshan Bot Garden, Shanghai Key Lab Plant Funct Genom & Resources, Shanghai 201602, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 4.Chinese Acad Sci, CAS Ctr Excellence Mol Plant Sci, State Key Lab Plant Mol Genet, Shanghai Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 5.Shanghai Normal Univ, Sch Life Sci, Shanghai 200234, Peoples R China 6.Univ Calif Riverside, Dept Chem & Environm Engn, Riverside, CA 92521 USA 7.China Acad Chinese Med Sci, Natl Resource Ctr Chinese Mat Med, State Key Lab Dao Di Herbs, Beijing 100700, Peoples R China
推荐引用方式 GB/T 7714	Song,Jiao-Jiao,Fang,Xin,Li,Chen-Yi,et al. A 2-oxoglutarate-dependent dioxygenase converts dihydrofuran to furan in Salvia diterpenoids[J]. PLANT PHYSIOLOGY,2022,188(3):1496-1506.
APA	Song,Jiao-Jiao.,Fang,Xin.,Li,Chen-Yi.,Jiang,Yan.,Li,Jian-Xu.,...&Yang,Lei.(2022).A 2-oxoglutarate-dependent dioxygenase converts dihydrofuran to furan in Salvia diterpenoids.PLANT PHYSIOLOGY,188(3),1496-1506.
MLA	Song,Jiao-Jiao,et al."A 2-oxoglutarate-dependent dioxygenase converts dihydrofuran to furan in Salvia diterpenoids".PLANT PHYSIOLOGY 188.3(2022):1496-1506.