AmAT19, an acetyltransferase from Astragalus membranaceus, catalyses specific 6 alpha-OH acetylation for tetracyclic triterpenes and steroids

doi:10.1039/d1ob01106e

KIB OpenIR

	AmAT19, an acetyltransferase from Astragalus membranaceus, catalyses specific 6 alpha-OH acetylation for tetracyclic triterpenes and steroids
	Wang,Linlin; Chen,Kuan ; Wang,Zilong ; Yi,Yang ; Zhang,Meng ; Hasan,Aobulikasimu ; Kuang,Yi ; Shaker,Sharpkate ; Yu,Rong ; Wang,Haotian ; Liu,Haiyang; Ye,Min ; Qiao,Xue
	2021
发表期刊	ORGANIC & BIOMOLECULAR CHEMISTRY
ISSN	1477-0520
卷号	19 期号:33 页码:7186-7189
摘要	Tetracyclic triterpenes and steroids are pharmacologically important molecules, and acetylation could improve their bioactivities. In this study, a highly regio- and stereo-specific acetyltransferase, AmAT19, was discovered from Astragalus membranaceus. AmAT19 could selectively catalyze the 6 alpha-OH acetylation of four tetracyclic triterpenes and steroids. The strict selectivity is associated with different orientations of the 6 alpha/beta-OH as indicated by molecular docking. Acetylated products 1a, 3a and 4a remarkably increased the inhibitory activity against the 3-chymotrypsin-like protease (3CL(pro)) of SARS-CoV-2, compared to 1, 3, and 4. AmAT19 could be a promising catalyst for specific 6 alpha-OH acetylation to expand the molecular diversity of triterpenes and steroids.
关键词	ACYLATION ACYLTRANSFERASE CYCLOASTRAGENOL BAHD
DOI	10.1039/d1ob01106e
WOS记录号	WOS:000683435600001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73123
专题	中国科学院昆明植物研究所
作者单位	1.Peking Univ, Sch Pharmaceut Sci, State Key Lab Nat & Biomimet Drugs, 38 Xueyuan Rd, Beijing 100191, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Nat Med Chem, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Wang,Linlin,Chen,Kuan,Wang,Zilong,et al. AmAT19, an acetyltransferase from Astragalus membranaceus, catalyses specific 6 alpha-OH acetylation for tetracyclic triterpenes and steroids[J]. ORGANIC & BIOMOLECULAR CHEMISTRY,2021,19(33):7186-7189.
APA	Wang,Linlin.,Chen,Kuan.,Wang,Zilong.,Yi,Yang.,Zhang,Meng.,...&Qiao,Xue.(2021).AmAT19, an acetyltransferase from Astragalus membranaceus, catalyses specific 6 alpha-OH acetylation for tetracyclic triterpenes and steroids.ORGANIC & BIOMOLECULAR CHEMISTRY,19(33),7186-7189.
MLA	Wang,Linlin,et al."AmAT19, an acetyltransferase from Astragalus membranaceus, catalyses specific 6 alpha-OH acetylation for tetracyclic triterpenes and steroids".ORGANIC & BIOMOLECULAR CHEMISTRY 19.33(2021):7186-7189.