Knowledge Management System of Kunming Institute of Botany,CAS
| The transcription factor WRKY75 positively regulates jasmonate-mediated plant defense to necrotrophic fungal pathogens | |
| Chen,Ligang; Zhang,Liping; Xiang,Shengyuan; Chen,Yanli; Zhang,Haiyan; Yu,Diqiu | |
| 2021 | |
| 发表期刊 | JOURNAL OF EXPERIMENTAL BOTANY
 |
| ISSN | 0022-0957 |
| 卷号 | 72期号:4页码:1473-1489 |
| 摘要 | Necrotrophic fungi cause devastating diseases in both horticultural and agronomic crops, but our understanding of plant defense responses against these pathogens is still limited. In this study, we demonstrated that WRKY75 positively regulates jasmonate (JA)-mediated plant defense against necrotrophic fungal pathogens Botrytis cinerea and Alternaria brassicicola, and also affects the sensitivity of plants to JA-inhibited seed germination and root growth. Quantitative analysis indicated that several JA-associated genes, such as OCTADECANOID-RESPONSIVE ARABIDOPSIS (ORA59) and PLANT DEFENSIN 1.2A (PDF1.2), were significantly reduced in expression in wrky75 mutants, and enhanced in WRKY75 overexpressing transgenic plants. Immunoprecipitation assays revealed that WRKY75 directly binds to the promoter of ORA59 and represses itstranscription. In vivo and in vitro experiments suggested that WRKY75 interacts with several JASMONATE ZIM-domain proteins, repressors of the JA signaling pathway. We determined that JASMONATE-ZIM-DOMAIN PROTEIN 8 (JAZ8) represses the transcriptional function of WRKY75, thereby attenuating the expression of its regulation. Overexpression of JAZ8 repressed plant defense responses to B. cinerea. Our study provides evidence that WRKY75 functions as a critical component of the JA-mediated signaling pathway to positively regulate Arabidopsis defense responses to necrotrophic pathogens. |
| 关键词 | Botrytis cinerea JA-associated genes jasmonate JAZ necrotrophic pathogen WRKY75 DOMAIN PROTEINS INTERACT SALICYLIC-ACID PHOSPHATE ACQUISITION SEED-GERMINATION JAZ REPRESSORS ARABIDOPSIS RESISTANCE EXPRESSION RESPONSES HORMONE |
| DOI | 10.1093/jxb/eraa529 |
| WOS记录号 | WOS:000637289700038 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/73118 |
| 专题 | 中国科学院昆明植物研究所 |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Trop Plant Resources & Sustainable Us, Xishuangbanna Trop Bot Garden, Mengla 666303, Yunnan, Peoples R China 2.Chinese Acad Sci, Ctr Econ Bot, Core Bot Gardens, Mengla 666303, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, CAS Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen,Ligang,Zhang,Liping,Xiang,Shengyuan,et al. The transcription factor WRKY75 positively regulates jasmonate-mediated plant defense to necrotrophic fungal pathogens[J]. JOURNAL OF EXPERIMENTAL BOTANY,2021,72(4):1473-1489. |
| APA | Chen,Ligang,Zhang,Liping,Xiang,Shengyuan,Chen,Yanli,Zhang,Haiyan,&Yu,Diqiu.(2021).The transcription factor WRKY75 positively regulates jasmonate-mediated plant defense to necrotrophic fungal pathogens.JOURNAL OF EXPERIMENTAL BOTANY,72(4),1473-1489. |
| MLA | Chen,Ligang,et al."The transcription factor WRKY75 positively regulates jasmonate-mediated plant defense to necrotrophic fungal pathogens".JOURNAL OF EXPERIMENTAL BOTANY 72.4(2021):1473-1489. |
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| 54.The transcription（8072KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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