Distinct Compartmentalization of Microbial Community and Potential Metabolic Function in the Fruiting Body of Tricholoma matsutake

doi:10.3390/jof7080586

KIB OpenIR

	Distinct Compartmentalization of Microbial Community and Potential Metabolic Function in the Fruiting Body of Tricholoma matsutake
	Liu,Dong ; Perez-Moreno,Jesus ; Zhang,Peng ; Wang,Ran ; Chater,Caspar C. C.; Yu,Fuqiang
	2021
发表期刊	JOURNAL OF FUNGI
ISSN	2309-608X
卷号	7 期号:8 页码:586
摘要	The uniquely compartmentalized fruiting body structure of the ectomycorrhizal fungus (EMF) Tricholoma matsutake, is a hotspot of microbial habitation and interaction. However, microbial diversity within this microniche structure of the EMF is rarely investigated. Furthermore, there is limited information concerning microbiomes associated with sporomes belonging to the ubiquitous fungal phylum Basidiomycota, particularly with respect to fungus-EMF interactions. In this study, we conducted high throughput sequencing, using ITS (fungal) and 16S rRNA (bacterial) marker genes to characterize and compare fruiting body microbiomes in the outer (pileipellis and stipitipellis) and inner layers (pileum context, stipe context, and lamellae) of the fruiting body of T. matsutake. Our results show the number of unique bacterial operational taxonomic units (OTUs) among the different compartments ranged from 410 to 499 and was more than double that of the shared/common OTUs (235). Micrococcales, Bacillales, Caulobacter, and Sphingomonas were the primary significant bacterial taxa within the different compartments of the dissected T. matsutake fruiting body. Non-parametric multivariate analysis of variance showed significant compartmental differences for both the bacterial and the fungal community structure within the T. matsutake fruiting body. The metabolic profiling revealed putative metabolisms (of amino acids, carbohydrates, and nucleotides) and the biosynthesis of secondary metabolites to be highly enriched in outer layers; in the inner parts, the metabolisms of energy, cofactors, vitamins, and lipids were significantly higher. This study demonstrates for the first time the distinct compartmentalization of microbial communities and potential metabolic function profiles in the fruiting body of an economically important EMF T. matsutake.
关键词	Tricholoma matsutake ectomycorrhizal fungi microbial partitioning metabolic profiling Basidiomycota fruiting body TUBER-PSEUDOBRUMALE VOLATILE COMPOUNDS GENE TRUFFLES BODIES FUNGI
DOI	10.3390/jof7080586
WOS记录号	WOS:000689617100001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73115
专题	中国科学院昆明植物研究所
作者单位	1.Chinese Acad Sci, Germplasm Bank Wild Species, Yunnan Key Lab Fungal Divers & Green Dev, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China 2.Colegio Postgrad, Campus Montecillo, Edafologia 56230, Texcoco, Mexico 3.Univ Lleida, Dept Crop & Forest Sci, Av Alcalde Rovira Roure 191, Lleida 25198, Spain 4.Chater, Caspar C. C.] Royal Bot Gardens, Dept Nat Capital & Plant Hlth, Richmond TW9 3AE, Surrey, England
推荐引用方式 GB/T 7714	Liu,Dong,Perez-Moreno,Jesus,Zhang,Peng,et al. Distinct Compartmentalization of Microbial Community and Potential Metabolic Function in the Fruiting Body of Tricholoma matsutake[J]. JOURNAL OF FUNGI,2021,7(8):586.
APA	Liu,Dong,Perez-Moreno,Jesus,Zhang,Peng,Wang,Ran,Chater,Caspar C. C.,&Yu,Fuqiang.(2021).Distinct Compartmentalization of Microbial Community and Potential Metabolic Function in the Fruiting Body of Tricholoma matsutake.JOURNAL OF FUNGI,7(8),586.
MLA	Liu,Dong,et al."Distinct Compartmentalization of Microbial Community and Potential Metabolic Function in the Fruiting Body of Tricholoma matsutake".JOURNAL OF FUNGI 7.8(2021):586.