New cytotoxic dichapetalins in the leaves of Phyllanthus acidus: Identification, quantitative analysis, and preliminary toxicity assessment

doi:10.1016/j.bioorg.2021.105125

KIB OpenIR

	New cytotoxic dichapetalins in the leaves of Phyllanthus acidus: Identification, quantitative analysis, and preliminary toxicity assessment
	Geng,Hui-Chun ; Zhu,Hong-Tao ; Yang,Wei-Nong ; Wang,Dong; Yang,Chong-Ren ; Zhang,Ying-Jun
	2021
发表期刊	BIOORGANIC CHEMISTRY
ISSN	0045-2068
卷号	114 页码:105125
摘要	The young leaves of Phyllanthus acidus (Euphorbiaceae) are commonly used as edible vegetables in Indonesia, Thailand, and India, and their water infusions as dieting aids for people trying to remain slim. However, it is regarded as a poisonous plant in Malaya, and current researches are insufficient to provide a conclusion on its toxicity and safety under large doses. In this study, we firstly found that the refined nonpolar extracts of P. acidus leaves showed significant cytotoxic effect against BEAS-2B and L02 normal cell lines with IC50 values of 2.15 and 1.64 mg/mL, respectively. Further bioactivity-guided isolation produced four new rare dichapetalins (pacidusins A-D) from the most active fraction. Their structures including absolute configurations were elucidated by extensive spectroscopic data and X-ray diffraction analysis. All the isolated dichapetalins exhibited moderate cytotoxicity against, BEAS-2B and L02 normal cell lines with IC50 values ranging from 12.44 to 22.55 mu M, as well as five human cancer cell lines with IC50 values ranging from 3.38 to 22.38 mu M. Furthermore, the content of the main dichapetalins in the leaves were determined by analytical HPLC, which showed that the leaves contained a very high amount of the four isolated dichapetalins with a total yield of 0.488 mg/g of dry plant material. These toxic dichapetalins may lead to adverse health effects in higher doses. Our findings indicate that the dichapetalin containing leaves may not be suitable for consumption in large quantities as food, but demonstrate their potency as anti-cancer agents for new drug discovery.
关键词	Phyllanthus acidus Euphorbiaceae Dichapetalins Pacidusins A-D Cytotoxicity TRITERPENOIDS CONSTITUENTS
DOI	10.1016/j.bioorg.2021.105125
WOS记录号	WOS:000691056700003
引用统计	被引频次：9[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73040
专题	中国科学院昆明植物研究所
作者单位	1.[Geng, Hui-Chun 2.Zhu, Hong-Tao 3.Wang, Dong 4.Yang, Chong-Ren 5.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 6.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 7.Yunnan Xinxing Greening Engn Co, Kunming 650228, Yunnan, Peoples R China 8.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Nat Med Chem, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Geng,Hui-Chun,Zhu,Hong-Tao,Yang,Wei-Nong,et al. New cytotoxic dichapetalins in the leaves of Phyllanthus acidus: Identification, quantitative analysis, and preliminary toxicity assessment[J]. BIOORGANIC CHEMISTRY,2021,114:105125.
APA	Geng,Hui-Chun,Zhu,Hong-Tao,Yang,Wei-Nong,Wang,Dong,Yang,Chong-Ren,&Zhang,Ying-Jun.(2021).New cytotoxic dichapetalins in the leaves of Phyllanthus acidus: Identification, quantitative analysis, and preliminary toxicity assessment.BIOORGANIC CHEMISTRY,114,105125.
MLA	Geng,Hui-Chun,et al."New cytotoxic dichapetalins in the leaves of Phyllanthus acidus: Identification, quantitative analysis, and preliminary toxicity assessment".BIOORGANIC CHEMISTRY 114(2021):105125.

条目包含的文件		下载所有文件
文件名称/大小	文献类型	版本类型	开放类型	使用许可
034-C07-197-张颖君组-BC.（774KB）	期刊论文	出版稿	开放获取	CC BY-NC-SA	浏览下载