High-molecular-weight fucosylated glycosaminoglycan induces human platelet aggregation depending on alpha(IIb)beta(3) and platelet secretion

doi:10.1080/09537104.2020.1820976

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	High-molecular-weight fucosylated glycosaminoglycan induces human platelet aggregation depending on alpha(IIb)beta(3) and platelet secretion
	Lin,Lisha ; Yang,Lian ; Chen,Jun ; Zhou,Lutan ; Li,Sujuan ; Gao,Na ; Zhao,Jinhua
	2021
发表期刊	PLATELETS
ISSN	0953-7104
卷号	32 期号:7 页码:975-983
摘要	Fucosylated glycosaminoglycan (FG) from sea cucumbers has been reported to have anticoagulant effects via targeting intrinsic tenase. However, FG from natural source also potentially poses risks due to its FXIIa activation and platelet aggregating effects. Here, we found that the effect of FG on human platelet aggregation depended on both the sulfation pattern and chain length. FGs with higher content of Fuc(2S4S) and larger molecular weight (>= 14 kD) had stronger activity. Both platelet aggregation and P-selectin expression induced by TaFG (an FG fromThelenota ananas) were decreased as the molecular weight reduced. Ticagrelor, aspirin and wortmannin completely blocked the secretion (ADP) but only partially blocked the aggregation induced by TaFG. Tirofiban an alpha(IIb)beta(3) antagonist however potently inhibited both the secretion and aggregation, with IC50 of 6.01 +/- 1.1.97 nM. Furthermore, TaFG could bind to human alpha(IIb)beta(3) with high affinity, and the affinities of two FGs were paralleled with their activity in platelet aggregation or activation. These results indicated that alpha(IIb)beta(3) played an important role in TaFG-induced platelet aggregation which was mediated by PI3K, and that platelet secretion was required for the amplification of aggregation.
关键词	Aggregation fucosylated glycosaminoglycan human platelets secretion alpha(IIb beta 3) DEPOLYMERIZED HOLOTHURIAN GLYCOSAMINOGLYCAN INTRINSIC TENASE COMPLEX INTEGRIN ALPHA(IIB)BETA(3) ACTIVATION AGGLUTINATION RECEPTOR ADP INHIBITION GENERATION MECHANISM
DOI	10.1080/09537104.2020.1820976
WOS记录号	WOS:000572529300001
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/73028
专题	中国科学院昆明植物研究所
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Yunnan Univ, Sch Chem Sci & Technol, Kunming 650091, Yunnan, Peoples R China 4.South Cent Univ Nationalities, Sch Pharmaceut Sci, Wuhan 430074, Peoples R China
推荐引用方式 GB/T 7714	Lin,Lisha,Yang,Lian,Chen,Jun,et al. High-molecular-weight fucosylated glycosaminoglycan induces human platelet aggregation depending on alpha(IIb)beta(3) and platelet secretion[J]. PLATELETS,2021,32(7):975-983.
APA	Lin,Lisha.,Yang,Lian.,Chen,Jun.,Zhou,Lutan.,Li,Sujuan.,...&Zhao,Jinhua.(2021).High-molecular-weight fucosylated glycosaminoglycan induces human platelet aggregation depending on alpha(IIb)beta(3) and platelet secretion.PLATELETS,32(7),975-983.
MLA	Lin,Lisha,et al."High-molecular-weight fucosylated glycosaminoglycan induces human platelet aggregation depending on alpha(IIb)beta(3) and platelet secretion".PLATELETS 32.7(2021):975-983.

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