Discovery of novel modulators targeting human TRPC5: Docking-based virtual screening, molecular dynamics simulation and binding af fi nity predication

doi:10.1016/j.jmgm.2020.107795

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	Discovery of novel modulators targeting human TRPC5: Docking-based virtual screening, molecular dynamics simulation and binding af fi nity predication
	Liu,Bin ; Zhang,Wei ; Guo,Sheng ; Zuo,Zhili
	2021
发表期刊	JOURNAL OF MOLECULAR GRAPHICS & MODELLING
ISSN	1093-3263
卷号	102 页码:107795
摘要	Canonical transient receptor potential channel 5 (TRPC5) plays a key role in the regulation of central nervous system, cardiovascular system, kidney disease, cancer, and could be also involved in liver function, arthritis, diabetes-associated complications and so on. However, evidence of TRPC5 function on cellular or organismic levels is sparse. There is still a need for identifying novel and efficient TRPC5 channel modulators to study TRPC5 function. In this study, based on the hTRPC5 structure obtained by homology modeling and the predicted binding site, we have performed virtual screening of 212,736 compounds from the specs database(http://www.specs.net) to find potential hTRPC5 modulators. Lipinski and Veber rules, ADMET (Absorption, Distribution, Metabolism, Excretion, Toxicity) and PAINS (Pan Assay Interference structures) filters were used to screen the large database. Further, multi-software combination docking, cluster analysis and interaction analysis were used to select 20 potential active candidates with novel skeleton. 4 Hits, bearing appreciable binding affinity with hTRPC5 were selected for 40ns all-atom molecular dynamics (MD) simulations under explicit water conditions. The MD simulation results suggested that the 4 Hits binding induces a slight structural change and stabilizes the hTRPC5 structure. In addition, decomposition free energy demonstrated that residues TRP434, LEU437, MET438, ALA441, ILE484, ILE487, LEU488, LEU491, LEU515, ILE517, LEU518, LEU521, PHE531, THR607, VAL610, ILE611, VAL615 played the critical role on system stability. 4 Hits, as potential modulators of hTRPC5, may be potential leads to develop effective therapeutics hTRPC5-associated diseases. (c) 2020 Elsevier Inc. All rights reserved.
关键词	hTRPC5 Homology modeling Molecular docking Molecular dynamics simulation Virtual screening INHIBITOR CHANNELS GROMACS POTENT AMBER IDENTIFICATION CONSTRAINTS INTEGRATION AMYGDALA
DOI	10.1016/j.jmgm.2020.107795
WOS记录号	WOS:000594996900002
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/72998
专题	中国科学院昆明植物研究所
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Liu,Bin,Zhang,Wei,Guo,Sheng,et al. Discovery of novel modulators targeting human TRPC5: Docking-based virtual screening, molecular dynamics simulation and binding af fi nity predication[J]. JOURNAL OF MOLECULAR GRAPHICS & MODELLING,2021,102:107795.
APA	Liu,Bin,Zhang,Wei,Guo,Sheng,&Zuo,Zhili.(2021).Discovery of novel modulators targeting human TRPC5: Docking-based virtual screening, molecular dynamics simulation and binding af fi nity predication.JOURNAL OF MOLECULAR GRAPHICS & MODELLING,102,107795.
MLA	Liu,Bin,et al."Discovery of novel modulators targeting human TRPC5: Docking-based virtual screening, molecular dynamics simulation and binding af fi nity predication".JOURNAL OF MOLECULAR GRAPHICS & MODELLING 102(2021):107795.

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