Assembly and comparative analyses of the mitochondrial genome of Castanospermum australe (Papilionoideae, Leguminosae)

doi:10.1071/SB19014

	Assembly and comparative analyses of the mitochondrial genome of Castanospermum australe (Papilionoideae, Leguminosae)
	Zhang, Rong 1,2; Jin, Jian-Jun 1; Moore, Michael J.3; Yi, Ting-Shuang1
通讯作者	Yi, Ting-Shuang(tingshuangyi@mail.kib.ac.cn)
	2019
发表期刊	AUSTRALIAN SYSTEMATIC BOTANY
ISSN	1030-1887
卷号	32 期号:5-6 页码:484-494
摘要	Plant mitochondrial genomes are often difficult to assemble because of frequent recombination mediated by repeats. Only a few mitochondrial genomes have been characterised in subfamily Papilionoideae of Leguminosae. Here, we report the complete mitochondrial genome of Castanospermum australe A.Cunn. & C.Fraser, an important medicinal and ornamental species in the Aldinoid clade of Papilionoideae. By mapping paired-end reads, seven hypothetical subgenomic conformations were rejected and two hypothetical complete isometric mitochondrial genome conformations that differed by a 64-kb inversion were strongly supported. Quantitative assessment of repeat-spanning read pairs showed a major conformation (MC1) and a minor conformation (MC2). The complete mitochondrial genome of C. australe was, thus, generated as 542 079 bp in length, with a high depth of coverage (similar to 389.7x). Annotation of this mitochondrial genome yielded 58 genes encoding 37 proteins, 18 tRNAs and three rRNAs, as well as 17 introns and three medium-sized repeats (133, 119 and 114 bp). Comparison of 10 mitochondrial genomes from Papilionoideae demonstrated significant variation in genome size, structure, gene content and RNA editing sites. In addition, mitochondrial genes were shown to be potentially useful in resolving the deep relationships of Papilionoideae.
关键词	genome skimming legume mitochondrion Moreton Bay chestnut phylogeny read-pair mapping
DOI	10.1071/SB19014
收录类别	SCI ; SCI
语种	英语
WOS记录号	WOS:000493529900006
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/70949
专题	中国西南野生生物种质资源库
通讯作者	Yi, Ting-Shuang
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Lanhei Rd, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Yuquan Rd, Beijing 100049, Peoples R China 3.Oberlin Coll, Dept Biol, Coll St, Oberlin, OH 44074 USA
推荐引用方式 GB/T 7714	Zhang, Rong,Jin, Jian-Jun,Moore, Michael J.,et al. Assembly and comparative analyses of the mitochondrial genome of Castanospermum australe (Papilionoideae, Leguminosae)[J]. AUSTRALIAN SYSTEMATIC BOTANY,2019,32(5-6):484-494.
APA	Zhang, Rong,Jin, Jian-Jun,Moore, Michael J.,&Yi, Ting-Shuang.(2019).Assembly and comparative analyses of the mitochondrial genome of Castanospermum australe (Papilionoideae, Leguminosae).AUSTRALIAN SYSTEMATIC BOTANY,32(5-6),484-494.
MLA	Zhang, Rong,et al."Assembly and comparative analyses of the mitochondrial genome of Castanospermum australe (Papilionoideae, Leguminosae)".AUSTRALIAN SYSTEMATIC BOTANY 32.5-6(2019):484-494.

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