Time-coursed transcriptome analysis identifies key expressional regulation in growth cessation and dormancy induced by short days in Paulownia
Wang, Jiayuan1; Wang, Hongyan2; Deng, Tao3; Liu, Zhen1; Wang, Xuewen3,4
Corresponding AuthorLiu, Zhen(liuzh20@163.com) ; Wang, Xuewen(xwwang@uga.edu)
2019-11-12
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume9Pages:14
AbstractMaintaining the viability of the apical shoot is critical for continued vertical growth in plants. Terminal shoot of tree species Paulownia cannot regrow in subsequent years. The short day (SD) treatment leads to apical growth cessation and dormancy. To understand the molecular basis of this, we further conducted global RNA-Seq based transcriptomic analysis in apical shoots to check regulation of gene expression. We obtained similar to 219 million paired-end 125-bp Illumina reads from five time-courses and de novo assembled them to yield 49,054 unigenes. Compared with the untreated control, we identified 1540 differentially expressed genes (DEGs) which were found to involve in 116 metabolic pathways. Expression of 87% of DEGs exhibited switch-on or switch-off pattern, indicating key roles in growth cessation. Most DEGs were enriched in the biological process of gene ontology categories and at later treatment stages. The pathways of auxin and circadian network were most affected and the expression of associated DEGs was characterised. During SD induction, auxin genes IAA, ARF and SAURs were down-regulated and circadian genes including PIF3 and PRRS were up-regulated. PEPC in photosynthesis was constitutively upregulated, suggesting a still high CO2 concentrating activity; however, the converting CO2 to G3P in the Calvin cycle is low, supported by reduced expression of GAPDH encoding the catalysing enzyme for this step. This indicates a de-coupling point in the carbon fixation. The results help elucidate the molecular mechanisms for SD inducing dormancy and cessation in apical shoots.
DOI10.1038/s41598-019-53283-2
Indexed BySCI ; SCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000495857600056
Citation statistics
Cited Times:1[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/70946
Collection中国科学院东亚植物多样性与生物地理学重点实验室
Corresponding AuthorLiu, Zhen; Wang, Xuewen
Affiliation1.Henan Agr Univ, Coll Forestry, Zhengzhou 450002, Henan, Peoples R China
2.Liaoning Univ, Sch Iife Sci, Shenyang 110000, Liaoning, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China
4.Univ Georgia, Dept Genet, Athens, GA 30602 USA
Recommended Citation
GB/T 7714
Wang, Jiayuan,Wang, Hongyan,Deng, Tao,et al. Time-coursed transcriptome analysis identifies key expressional regulation in growth cessation and dormancy induced by short days in Paulownia[J]. SCIENTIFIC REPORTS,2019,9:14.
APA Wang, Jiayuan,Wang, Hongyan,Deng, Tao,Liu, Zhen,&Wang, Xuewen.(2019).Time-coursed transcriptome analysis identifies key expressional regulation in growth cessation and dormancy induced by short days in Paulownia.SCIENTIFIC REPORTS,9,14.
MLA Wang, Jiayuan,et al."Time-coursed transcriptome analysis identifies key expressional regulation in growth cessation and dormancy induced by short days in Paulownia".SCIENTIFIC REPORTS 9(2019):14.
Files in This Item: Download All
File Name/Size DocType Version Access License
Wang-2019-Time-cours(4089KB)期刊论文出版稿开放获取CC BY-NC-SAView Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[Wang, Jiayuan]'s Articles
[Wang, Hongyan]'s Articles
[Deng, Tao]'s Articles
Baidu academic
Similar articles in Baidu academic
[Wang, Jiayuan]'s Articles
[Wang, Hongyan]'s Articles
[Deng, Tao]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[Wang, Jiayuan]'s Articles
[Wang, Hongyan]'s Articles
[Deng, Tao]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: Wang-2019-Time-coursed transcriptome analysis.pdf
Format: Adobe PDF
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.