Time-coursed transcriptome analysis identifies key expressional regulation in growth cessation and dormancy induced by short days in Paulownia

doi:10.1038/s41598-019-53283-2

	Time-coursed transcriptome analysis identifies key expressional regulation in growth cessation and dormancy induced by short days in Paulownia
	Wang, Jiayuan 1; Wang, Hongyan 2; Deng, Tao3 ; Liu, Zhen 1; Wang, Xuewen 3,4
通讯作者	Liu, Zhen(liuzh20@163.com) ; Wang, Xuewen(xwwang@uga.edu)
	2019-11-12
发表期刊	SCIENTIFIC REPORTS
ISSN	2045-2322
卷号	9 页码:14
摘要	Maintaining the viability of the apical shoot is critical for continued vertical growth in plants. Terminal shoot of tree species Paulownia cannot regrow in subsequent years. The short day (SD) treatment leads to apical growth cessation and dormancy. To understand the molecular basis of this, we further conducted global RNA-Seq based transcriptomic analysis in apical shoots to check regulation of gene expression. We obtained similar to 219 million paired-end 125-bp Illumina reads from five time-courses and de novo assembled them to yield 49,054 unigenes. Compared with the untreated control, we identified 1540 differentially expressed genes (DEGs) which were found to involve in 116 metabolic pathways. Expression of 87% of DEGs exhibited switch-on or switch-off pattern, indicating key roles in growth cessation. Most DEGs were enriched in the biological process of gene ontology categories and at later treatment stages. The pathways of auxin and circadian network were most affected and the expression of associated DEGs was characterised. During SD induction, auxin genes IAA, ARF and SAURs were down-regulated and circadian genes including PIF3 and PRRS were up-regulated. PEPC in photosynthesis was constitutively upregulated, suggesting a still high CO2 concentrating activity; however, the converting CO2 to G3P in the Calvin cycle is low, supported by reduced expression of GAPDH encoding the catalysing enzyme for this step. This indicates a de-coupling point in the carbon fixation. The results help elucidate the molecular mechanisms for SD inducing dormancy and cessation in apical shoots.
DOI	10.1038/s41598-019-53283-2
收录类别	SCI ; SCI
语种	英语
WOS记录号	WOS:000495857600056
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/70946
专题	中国科学院东亚植物多样性与生物地理学重点实验室
通讯作者	Liu, Zhen; Wang, Xuewen
作者单位	1.Henan Agr Univ, Coll Forestry, Zhengzhou 450002, Henan, Peoples R China 2.Liaoning Univ, Sch Iife Sci, Shenyang 110000, Liaoning, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming 650201, Yunnan, Peoples R China 4.Univ Georgia, Dept Genet, Athens, GA 30602 USA
推荐引用方式 GB/T 7714	Wang, Jiayuan,Wang, Hongyan,Deng, Tao,et al. Time-coursed transcriptome analysis identifies key expressional regulation in growth cessation and dormancy induced by short days in Paulownia[J]. SCIENTIFIC REPORTS,2019,9:14.
APA	Wang, Jiayuan,Wang, Hongyan,Deng, Tao,Liu, Zhen,&Wang, Xuewen.(2019).Time-coursed transcriptome analysis identifies key expressional regulation in growth cessation and dormancy induced by short days in Paulownia.SCIENTIFIC REPORTS,9,14.
MLA	Wang, Jiayuan,et al."Time-coursed transcriptome analysis identifies key expressional regulation in growth cessation and dormancy induced by short days in Paulownia".SCIENTIFIC REPORTS 9(2019):14.

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