Enhanced Coproduction of Cell-Bound Zeaxanthin and Secreted Exopolysaccharides by Sphingobium sp. via Metabolic Engineering and Optimized Fermentation

doi:10.1021/acs.jafc.9b05342

	Enhanced Coproduction of Cell-Bound Zeaxanthin and Secreted Exopolysaccharides by Sphingobium sp. via Metabolic Engineering and Optimized Fermentation
	Liu, Mengmeng 1,2; Sandmann, Gerhard 3; Chen, Feng 4; Huang, Junchao1
通讯作者	Huang, Junchao(huangjc@mail.kib.ac.cn)
	2019-11-06
发表期刊	JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
ISSN	0021-8561
卷号	67 期号:44 页码:12228-12236
摘要	Zeaxanthin is a value-added carotenoid with wide applications. This study aims to manipulate a generally recognized as safe and carotenoid-producing bacterium, Sphingobium sp., for enhanced production of zeaxanthin and exopolysaccharides. First, whole-genome sequencing and analysis of pathway genes were applied to define the carotenoid pathway in Sphingobium sp. Second, a Sphingobium transformation system was established to engineer metabolite flux into zeaxanthin. By a combination of chemical mutagenesis and removal of bottlenecks of carotenoid biosynthesis via overexpression of three rate-limiting enzymes, the genetically modified Sphingobium DIZ strain produced 21.26 mg/g dry cell weight of zeaxanthin, which was about 4-fold higher than the wild type. Upon optimization of culture conditions, the DIZ strain produced 479.5 mg/L of zeaxanthin with the productivity of 4.99 mg/L/h and 21.9 g/L of exopolysaccharides using a fed-batch fermentation strategy. This study represents the first genetic manipulation of Sphingobium sp., a biotechnologically important bacterium, for high-yield production of value-added metabolites.
关键词	carotenoid metabolite zeaxanthin Sphingobium
DOI	10.1021/acs.jafc.9b05342
收录类别	SCI ; SCI
语种	英语
WOS记录号	WOS:000495769800010
引用统计	被引频次：10[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/70924
专题	资源植物与生物技术所级重点实验室
通讯作者	Huang, Junchao
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Goethe Univ Frankfurt, Inst Mol Biosci, Max von Laue Str 9, D-60438 Frankfurt, Germany 4.Shenzhen Univ, Inst Adv Study, Shenzhen 518060, Peoples R China
推荐引用方式 GB/T 7714	Liu, Mengmeng,Sandmann, Gerhard,Chen, Feng,et al. Enhanced Coproduction of Cell-Bound Zeaxanthin and Secreted Exopolysaccharides by Sphingobium sp. via Metabolic Engineering and Optimized Fermentation[J]. JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,2019,67(44):12228-12236.
APA	Liu, Mengmeng,Sandmann, Gerhard,Chen, Feng,&Huang, Junchao.(2019).Enhanced Coproduction of Cell-Bound Zeaxanthin and Secreted Exopolysaccharides by Sphingobium sp. via Metabolic Engineering and Optimized Fermentation.JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY,67(44),12228-12236.
MLA	Liu, Mengmeng,et al."Enhanced Coproduction of Cell-Bound Zeaxanthin and Secreted Exopolysaccharides by Sphingobium sp. via Metabolic Engineering and Optimized Fermentation".JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY 67.44(2019):12228-12236.

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