Comparative proteomic and transcriptomic analyses provide new insight into the formation of seed size in castor bean

doi:10.1186/s12870-020-2249-1

	Comparative proteomic and transcriptomic analyses provide new insight into the formation of seed size in castor bean
	Yu, Anmin 1,2; Li, Fei 2; Liu, Aizhong1
通讯作者	Liu, Aizhong(liuaizhong@mail.kib.ac.cn)
	2020-01-30
发表期刊	BMC PLANT BIOLOGY
ISSN	1471-2229
卷号	20 期号:1 页码:20
摘要	Background Little is known about the molecular basis of seed size formation in endospermic seed of dicotyledons. The seed of castor bean (Ricinus communis L.) is considered as a model system in seed biology studies because of its persistent endosperms throughout seed development. Results We compared the size of endosperm and endospermic cells between ZB107 and ZB306 and found that the larger seed size of ZB107 resulted from a higher cell count in the endosperm, which occupy a significant amount of the total seed volume. In addition, fresh weight, dry weight, and protein content of seeds were remarkably higher in ZB107 than in ZB306. Comparative proteomic and transcriptomic analyses were performed between large-seed ZB107 and small-seed ZB306, using isobaric tags for relative and absolute quantification (iTRAQ) and RNA-seq technologies, respectively. A total of 1416 protein species were identified, of which 173 were determined as differentially abundant protein species (DAPs). Additionally, there were 9545 differentially expressed genes (DEGs) between ZB306 and ZB107. Functional analyses revealed that these DAPs and DEGs were mainly involved in cell division and the metabolism of carbohydrates and proteins. Conclusions These findings suggest that both cell number and storage-component accumulation are critical for the formation of seed size, providing new insight into the potential mechanisms behind seed size formation in endospermic seeds.
DOI	10.1186/s12870-020-2249-1
收录类别	SCI ; SCI
语种	英语
WOS记录号	WOS:000513239400002
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/70872
专题	资源植物与生物技术所级重点实验室
通讯作者	Liu, Aizhong
作者单位	1.Southwest Forestry Univ, Key Lab Forest Resources Conservat & Utilizat Sou, Minist Educ, Kunming 650224, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Yu, Anmin,Li, Fei,Liu, Aizhong. Comparative proteomic and transcriptomic analyses provide new insight into the formation of seed size in castor bean[J]. BMC PLANT BIOLOGY,2020,20(1):20.
APA	Yu, Anmin,Li, Fei,&Liu, Aizhong.(2020).Comparative proteomic and transcriptomic analyses provide new insight into the formation of seed size in castor bean.BMC PLANT BIOLOGY,20(1),20.
MLA	Yu, Anmin,et al."Comparative proteomic and transcriptomic analyses provide new insight into the formation of seed size in castor bean".BMC PLANT BIOLOGY 20.1(2020):20.