Identification and Characterization of ShSPI, a Kazal-Type Elastase Inhibitor from the Venom of Scolopendra Hainanum
Luan, Ning1; Zhao, Qiyu2; Duan, Zilei2; Ji, Mengyao1; Xing, Meichen1; Zhu, Tengyu1; Mwangi, James2; Rong, Mingqiang3; Liu, Jiangxin4; Lai, Ren1,2,5
Corresponding AuthorRong, Mingqiang(rongmq@hunnu.edu.cn) ; Liu, Jiangxin(liujiangxin@mail.kib.ac.cn) ; Lai, Ren(rlai@mail.kiz.ac.cn)
2019-12-01
Source PublicationTOXINS
Volume11Issue:12Pages:13
AbstractElastase is a globular glycoprotein and belongs to the chymotrypsin family. It is involved in several inflammatory cascades on the basis of cleaving the important connective tissue protein elastin, and is strictly regulated to a balance by several endogenous inhibitors. When elastase and its inhibitors are out of balance, severe diseases will develop, especially those involved in the cardiopulmonary system. Much attention has been attracted in seeking innovative elastase inhibitors and various advancements have been taken on clinical trials of these inhibitors. Natural functional peptides from venomous animals have been shown to have anti-protease properties. Here, we identified a kazal-type serine protease inhibitor named ShSPI from the cDNA library of the venom glands of Scolopendra hainanum. ShSPI showed significant inhibitory effects on porcine pancreatic elastase and human neutrophils elastase with Ki values of 225.83 +/- 20 nM and 12.61 +/- 2 nM, respectively. Together, our results suggest that ShSPI may be an excellent candidate to develop a drug for cardiopulmonary diseases.
Keywordcentipede kazal-type inhibitor human neutrophils elastase ShSPI
DOI10.3390/toxins11120708
Indexed BySCI ; SCI
Language英语
WOS Research AreaFood Science & Technology ; Toxicology
WOS SubjectFood Science & Technology ; Toxicology
WOS IDWOS:000507337800031
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/70620
Collection植物化学与西部植物资源持续利用国家重点实验室
Corresponding AuthorRong, Mingqiang; Liu, Jiangxin; Lai, Ren
Affiliation1.Nanjing Agr Univ, Dept Zool, Coll Life Sci, Nanjing 210095, Peoples R China
2.Chinese Acad Sci, Kunming Inst Zool, Key Lab Anim Models & Human Dis Mech, Key Lab Bioact Peptides Yunnan Prov, Kunming 650223, Yunnan, Peoples R China
3.Hunan Normal Univ, Coll Life Sci, Natl Local Joint Engn Lab Anim Peptide Drug Dev, Changsha 410081, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
5.Chinese Acad Sci, Kunming Inst Zool, Sino African Joint Res Ctr, Kunming 650223, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Luan, Ning,Zhao, Qiyu,Duan, Zilei,et al. Identification and Characterization of ShSPI, a Kazal-Type Elastase Inhibitor from the Venom of Scolopendra Hainanum[J]. TOXINS,2019,11(12):13.
APA Luan, Ning.,Zhao, Qiyu.,Duan, Zilei.,Ji, Mengyao.,Xing, Meichen.,...&Lai, Ren.(2019).Identification and Characterization of ShSPI, a Kazal-Type Elastase Inhibitor from the Venom of Scolopendra Hainanum.TOXINS,11(12),13.
MLA Luan, Ning,et al."Identification and Characterization of ShSPI, a Kazal-Type Elastase Inhibitor from the Venom of Scolopendra Hainanum".TOXINS 11.12(2019):13.
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