Total saponins from Paris forrestii (Takht) H. Li. show the anticancer and RNA expression regulating effects on prostate cancer cells

doi:10.1016/j.biopha.2019.109674

	Total saponins from Paris forrestii (Takht) H. Li. show the anticancer and RNA expression regulating effects on prostate cancer cells
	Xia, Chengxing 1; Chen, Liu 4; Sun, Wanghong 5; Yan, Ruping 1; Xia, Mengyuan 2,3; Wang, Yuehu2,3 ; Yang, Delin 1
通讯作者	Wang, Yuehu(wangyuehu@mail.kib.ac.cn) ; Yang, Delin(ydelin@163.com)
	2020
发表期刊	BIOMEDICINE & PHARMACOTHERAPY
ISSN	0753-3322
卷号	121 页码:11
摘要	Paris forrestii is a unique plant found in Tibet and Yunnan, China, and total saponins from Paris forrestii (PCT3) contain anticancer steroid glycosides. RNA expression plays an important role in various biological processes. However, the cytotoxicity effects and mechanisms of PCT3 in relation to prostate cancer (PCa) cells have not yet been reported. In the present study, the antitumor activity of PCT3 on PCa cells was evaluated. PCT3 displayed potent anticancer effects toward PCa cells that were similar to the effects of pure saponins from P. forrestii, but PCT3 had less activity in suppressing the prostate epithelial cell line RWPE. Furthermore, using CCK-8 assays, Edu incorporation, colony formation assays, Annexin V/PI assays and western blotting, we found that treatment with 4 mu g/mL PCT3 significantly decreased proliferation and induced apoptosis in PCa cells. Using wound healing and transwell assays, we demonstrated that treatment with 2 mu g/mL PCT3 significantly suppressed the migration and invasion of PCa cells. To explore the molecular mechanisms behind the anticancer effect of PCT3, PCT3 (5 mu g/mL) treated and untreated PCa cells (LNCAP and PC3 cell lines) were analyzed using transcriptomics. Taking the commonly differentially expressed genes (log2FC > 0.585) in both cell lines, 41 mRNAs and 5 lncRNAs were eventually identified. Bioinformatics analysis (GO and KEGG analyses) revealed that some genes involved in classical cell proliferation and apoptosis pathways were aberrantly expressed after PCT3 treatment of PCa cells. By using q-PCR, the expression levels of NEAT1, MALAT1, TIPIN, LYAR, IQGAP3, GINS2, and ZGRF1 were validated as consistent with microarray data, suggesting that these genes might participate in the PCT3 anticancer effect. The present study suggests that PCT3 exhibits an anticancer effect on PCa and reveals some crucial lncRNAs and mRNAs that are involved in the anticancer mechanisms of PCT3 on Pca.
关键词	Prostate cancer PCT3 Saponins Anticancer effect RNA microarray
DOI	10.1016/j.biopha.2019.109674
收录类别	SCI ; SCI
语种	英语
WOS记录号	WOS:000500746200123
引用统计	被引频次：15[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/70061
专题	资源植物与生物技术所级重点实验室
通讯作者	Wang, Yuehu; Yang, Delin
作者单位	1.Kunming Med Univ, Affiliated Hosp 2, Dept Urol, Kunming 650101, Yunnan, Peoples R China 2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China 3.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China 4.Hunan Univ Med, Affiliated Hosp 1, Dept Urol, Changsha 410000, Hunan, Peoples R China 5.Zhuji Peoples Hosp Zhejiang Prov, Dept Urol, Zhuji 311800, Peoples R China
推荐引用方式 GB/T 7714	Xia, Chengxing,Chen, Liu,Sun, Wanghong,et al. Total saponins from Paris forrestii (Takht) H. Li. show the anticancer and RNA expression regulating effects on prostate cancer cells[J]. BIOMEDICINE & PHARMACOTHERAPY,2020,121:11.
APA	Xia, Chengxing.,Chen, Liu.,Sun, Wanghong.,Yan, Ruping.,Xia, Mengyuan.,...&Yang, Delin.(2020).Total saponins from Paris forrestii (Takht) H. Li. show the anticancer and RNA expression regulating effects on prostate cancer cells.BIOMEDICINE & PHARMACOTHERAPY,121,11.
MLA	Xia, Chengxing,et al."Total saponins from Paris forrestii (Takht) H. Li. show the anticancer and RNA expression regulating effects on prostate cancer cells".BIOMEDICINE & PHARMACOTHERAPY 121(2020):11.