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Total saponins from Paris forrestii (Takht) H. Li. show the anticancer and RNA expression regulating effects on prostate cancer cells
Xia, Chengxing1; Chen, Liu4; Sun, Wanghong5; Yan, Ruping1; Xia, Mengyuan2,3; Wang, Yuehu2,3; Yang, Delin1
Corresponding AuthorWang, Yuehu(wangyuehu@mail.kib.ac.cn) ; Yang, Delin(ydelin@163.com)
2020
Source PublicationBIOMEDICINE & PHARMACOTHERAPY
ISSN0753-3322
Volume121Pages:11
AbstractParis forrestii is a unique plant found in Tibet and Yunnan, China, and total saponins from Paris forrestii (PCT3) contain anticancer steroid glycosides. RNA expression plays an important role in various biological processes. However, the cytotoxicity effects and mechanisms of PCT3 in relation to prostate cancer (PCa) cells have not yet been reported. In the present study, the antitumor activity of PCT3 on PCa cells was evaluated. PCT3 displayed potent anticancer effects toward PCa cells that were similar to the effects of pure saponins from P. forrestii, but PCT3 had less activity in suppressing the prostate epithelial cell line RWPE. Furthermore, using CCK-8 assays, Edu incorporation, colony formation assays, Annexin V/PI assays and western blotting, we found that treatment with 4 mu g/mL PCT3 significantly decreased proliferation and induced apoptosis in PCa cells. Using wound healing and transwell assays, we demonstrated that treatment with 2 mu g/mL PCT3 significantly suppressed the migration and invasion of PCa cells. To explore the molecular mechanisms behind the anticancer effect of PCT3, PCT3 (5 mu g/mL) treated and untreated PCa cells (LNCAP and PC3 cell lines) were analyzed using transcriptomics. Taking the commonly differentially expressed genes (log2FC > 0.585) in both cell lines, 41 mRNAs and 5 lncRNAs were eventually identified. Bioinformatics analysis (GO and KEGG analyses) revealed that some genes involved in classical cell proliferation and apoptosis pathways were aberrantly expressed after PCT3 treatment of PCa cells. By using q-PCR, the expression levels of NEAT1, MALAT1, TIPIN, LYAR, IQGAP3, GINS2, and ZGRF1 were validated as consistent with microarray data, suggesting that these genes might participate in the PCT3 anticancer effect. The present study suggests that PCT3 exhibits an anticancer effect on PCa and reveals some crucial lncRNAs and mRNAs that are involved in the anticancer mechanisms of PCT3 on Pca.
KeywordProstate cancer PCT3 Saponins Anticancer effect RNA microarray
DOI10.1016/j.biopha.2019.109674
Indexed BySCI ; SCI
Language英语
WOS IDWOS:000500746200123
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/70061
Collection资源植物与生物技术所级重点实验室
Corresponding AuthorWang, Yuehu; Yang, Delin
Affiliation1.Kunming Med Univ, Affiliated Hosp 2, Dept Urol, Kunming 650101, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China
4.Hunan Univ Med, Affiliated Hosp 1, Dept Urol, Changsha 410000, Hunan, Peoples R China
5.Zhuji Peoples Hosp Zhejiang Prov, Dept Urol, Zhuji 311800, Peoples R China
Recommended Citation
GB/T 7714
Xia, Chengxing,Chen, Liu,Sun, Wanghong,et al. Total saponins from Paris forrestii (Takht) H. Li. show the anticancer and RNA expression regulating effects on prostate cancer cells[J]. BIOMEDICINE & PHARMACOTHERAPY,2020,121:11.
APA Xia, Chengxing.,Chen, Liu.,Sun, Wanghong.,Yan, Ruping.,Xia, Mengyuan.,...&Yang, Delin.(2020).Total saponins from Paris forrestii (Takht) H. Li. show the anticancer and RNA expression regulating effects on prostate cancer cells.BIOMEDICINE & PHARMACOTHERAPY,121,11.
MLA Xia, Chengxing,et al."Total saponins from Paris forrestii (Takht) H. Li. show the anticancer and RNA expression regulating effects on prostate cancer cells".BIOMEDICINE & PHARMACOTHERAPY 121(2020):11.
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