| A CRISPR/LbCas12a-based method for highly efficient multiplex gene editing in Physcomitrella patens | |
Pu, Xiaojun1,2 ; Liu, Lina1,2,3; Li, Ping1,2,3; Huo, Heqiang4; Dong, Xiumei1,2; Xie, Kabin5; Yang, Hong1,2,3; Liu, Li1,2
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| 通讯作者 | Liu, Li(liulia@mail.kib.ac.cn) |
| 2019-09-06 | |
| 发表期刊 | PLANT JOURNAL
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| ISSN | 0960-7412 |
| 页码 | 10 |
| 摘要 | Due to their high efficiency, specificity, and flexibility, programmable nucleases, such as those of the clustered regularly interspaced short palindromic repeats (CRISPR)/Cas12a (Cpf1) system, have greatly expanded the applicability of editing the genomes of various organisms. Genes from different gene families or genes with redundant functions in the same gene family can be examined by assembling multiple CRISPR RNAs (crRNAs) in a single vector. However, the activity and efficiency of CRISPR/Cas12a in the non-vascular plant Physcomitrella patens are largely unknown. Here, we demonstrate that LbCas12a together with its mature crRNA can target multiple loci simultaneously in P. patens with high efficiency via co-delivery of LbCas12a and a crRNA expression cassette in vivo. The mutation frequencies induced by CRISPR/LbCas12a at a single locus ranged from 26.5 to 100%, with diverse deletions being the most common type of mutation. Our method expands the repertoire of genome editing tools available for P. patens and facilitates the creation of loss-of-function mutants of multiple genes from different gene families. |
| 关键词 | CRISPR Cas12a CRISPR Cas9 genome editing Physcomitrella patens technical advance |
| DOI | 10.1111/tpj.14478 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000485845800001 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/68705 |
| 专题 | 资源植物与生物技术所级重点实验室 |
| 通讯作者 | Liu, Li |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China 2.Yunnan Key Lab Wild Plant Resources, Kunming 650201, Yunnan, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 4.Univ Florida, Dept Environm Hort, Mid Florida Res & Educ Ctr, Gainesville, FL 32703 USA 5.Huazhong Agr Univ, Natl Key Lab Crop Genet Improvement, Wuhan 430070, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | Pu, Xiaojun,Liu, Lina,Li, Ping,et al. A CRISPR/LbCas12a-based method for highly efficient multiplex gene editing in Physcomitrella patens[J]. PLANT JOURNAL,2019:10. |
| APA | Pu, Xiaojun.,Liu, Lina.,Li, Ping.,Huo, Heqiang.,Dong, Xiumei.,...&Liu, Li.(2019).A CRISPR/LbCas12a-based method for highly efficient multiplex gene editing in Physcomitrella patens.PLANT JOURNAL,10. |
| MLA | Pu, Xiaojun,et al."A CRISPR/LbCas12a-based method for highly efficient multiplex gene editing in Physcomitrella patens".PLANT JOURNAL (2019):10. |
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| pu2019.pdf（2446KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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