Petchiether A attenuates obstructive nephropathy by suppressing TGF-ss/Smad3 and NF-kappa B signalling
You, Yong-Ke1,2; Luo, Qi3; Wu, Wei-Feng1; Zhang, Jiao-Jiao4; Zhu, Hong-Jian5; Lao, Lixing1,2; Lan, Hui Y.6,7; Chen, Hai-Yong1,2; Cheng, Yong-Xian3,4
通讯作者Chen, Hai-Yong(haiyong@hku.hk) ; Cheng, Yong-Xian(yxcheng@szu.edu.cn)
2019-08-01
发表期刊JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
卷号23期号:8页码:5576-5587
摘要Obstructive nephropathy is the end result of a variety of diseases that block drainage from the kidney(s). Transforming growth factor-ss 1 (TGF-ss 1)/Smad3-driven renal fibrosis is the common pathogenesis of obstructive nephropathy. In this study, we identified petchiether A (petA), a novel small-molecule meroterpenoid from Ganoderma, as a potential inhibitor of TGF-ss 1-induced Smad3 phosphorylation. The obstructive nephropathy was induced by unilateral ureteral obstruction (UUO) in mice. Mice received an intraperitoneal injection of petA/vehicle before and after UUO or sham operation. An in vivo study revealed that petA protected against renal inflammation and fibrosis by reducing the infiltration of macrophages, inhibiting the expression of proinflammatory cytokines (interleukin-1 ss and tumour necrosis factor-alpha) and reducing extracellular matrix deposition (alpha-smooth muscle actin, collagen I and fibronectin) in the obstructed kidney of UUO mice; these changes were associated with suppression of Smad3 and NF-kappa B p65 phosphorylation. Petchiether A inhibited Smad3 phosphorylation in vitro and down-regulated the expression of the fibrotic marker collagen I in TGF-ss 1-treated renal epithelial cells. Further, we found that petA dose-dependently suppressed Smad3-responsive promoter activity, indicating that petA inhibits gene expression downstream of the TGF-ss/Smad3 signalling pathway. In conclusion, our findings suggest that petA protects against renal inflammation and fibrosis by selectively inhibiting TGF-ss/Smad3 signalling.
关键词fibrosis NF-kappa B obstructive nephropathy petchiether A TGF-ss/Smad3
DOI10.1111/jcmm.14454
收录类别SCI
语种英语
WOS记录号WOS:000481532600066
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/68353
专题植物化学与西部植物资源持续利用国家重点实验室
通讯作者Chen, Hai-Yong; Cheng, Yong-Xian
作者单位1.Univ Hong Kong, Li Ka Shing Fac Med, Sch Chinese Med, Hong Kong, Peoples R China
2.Univ Hong Kong, Shenzhen Hosp, Dept Chinese Med, Shenzhen, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming, Yunnan, Peoples R China
4.Shenzhen Univ, Hlth Sci Ctr, Sch Pharmaceut Sci, Guangdong Key Lab Genome Stabil & Dis Prevent, Shenzhen, Peoples R China
5.Univ Melbourne, Dept Surg, Melbourne, Vic, Australia
6.Chinese Univ Hong Kong, Dept Med & Therapeut, Hong Kong, Peoples R China
7.Chinese Univ Hong Kong, Li Ka Shing Inst Hlth Sci, Hong Kong, Peoples R China
推荐引用方式
GB/T 7714
You, Yong-Ke,Luo, Qi,Wu, Wei-Feng,et al. Petchiether A attenuates obstructive nephropathy by suppressing TGF-ss/Smad3 and NF-kappa B signalling[J]. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,2019,23(8):5576-5587.
APA You, Yong-Ke.,Luo, Qi.,Wu, Wei-Feng.,Zhang, Jiao-Jiao.,Zhu, Hong-Jian.,...&Cheng, Yong-Xian.(2019).Petchiether A attenuates obstructive nephropathy by suppressing TGF-ss/Smad3 and NF-kappa B signalling.JOURNAL OF CELLULAR AND MOLECULAR MEDICINE,23(8),5576-5587.
MLA You, Yong-Ke,et al."Petchiether A attenuates obstructive nephropathy by suppressing TGF-ss/Smad3 and NF-kappa B signalling".JOURNAL OF CELLULAR AND MOLECULAR MEDICINE 23.8(2019):5576-5587.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
You-2019-Petchiether(3491KB)期刊论文出版稿开放获取CC BY-NC-SA浏览 请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[You, Yong-Ke]的文章
[Luo, Qi]的文章
[Wu, Wei-Feng]的文章
百度学术
百度学术中相似的文章
[You, Yong-Ke]的文章
[Luo, Qi]的文章
[Wu, Wei-Feng]的文章
必应学术
必应学术中相似的文章
[You, Yong-Ke]的文章
[Luo, Qi]的文章
[Wu, Wei-Feng]的文章
相关权益政策
暂无数据
收藏/分享
文件名: You-2019-Petchiether A attenuates obstructive.pdf
格式: Adobe PDF
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。