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Genome-wide analysis of the callose enzyme families of fertile and sterile flower buds of the Chinese cabbage (Brassica rapa L. ssp. pekinensis)
Pu, Yanan1,2,3; Hou, Lingyun1; Guo, Yingqi4; Ullah, Ikram1; Yang, Yongping2; Yue, Yanling1
Corresponding AuthorYang, Yongping(yangyp@mail.kib.ac.cn) ; Yue, Yanling(yanling-yue@126.com)
2019-08-01
Source PublicationFEBS OPEN BIO
ISSN2211-5463
Volume9Issue:8Pages:1432-1449
AbstractCallose is a beta-1,3-glucan commonly found in higher plants that plays an important role in regulating plant pollen development. It is synthesized by glucan synthase-like (GSL) and is degraded by the enzyme endo-1,3-beta-glucosidase. However, genome-wide analyses of callose GSL and endo-1,3-beta-glucosidase enzymes in fertile and sterile flower buds of Chinese cabbage have not yet been reported. Here, we show that delayed callose degradation at the tetrad stage may be the main cause of microspore abortion in Chinese cabbage with nuclear sterility near-isogenic line '10L03'. Fifteen callose GSLs and 77 endo-1,3-beta-glucosidase enzymes were identified in Chinese cabbage. Phylogenetic, gene structural and chromosomal analyses revealed that the expansion occurred due to three polyploidization events of these two gene families. Expression pattern analysis showed that the GSL and endo-1,3-beta-glucosidase enzymes are involved in the development of various tissues and that the genes functionally diverged during long-term evolution. Relative gene expression analysis of Chinese cabbage flowers at different developmental stages showed that high expression of the synthetic enzyme BraA01g041620 and low expression of AtA6-homologous genes (BraA04g008040, BraA07g009320, BraA01g030220 and BraA03g040850) and two other genes (BraA10g020080 and BraA05g038340) for degrading enzymes in the meiosis and tetrad stages may cause nuclear sterility in the near-isogenic line '10L03'. Overall, our data provide an important foundation for comprehending the potential roles of the callose GSL and endo-1,3-beta-glucosidase enzymes in regulating pollen development in Chinese cabbage.
Keywordcallose Chinese cabbage enzyme family gene expression nuclear sterility
DOI10.1002/2211-5463.12685
Indexed BySCI
Language英语
WOS IDWOS:000476128600001
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/68242
Collection资源植物与生物技术所级重点实验室
Corresponding AuthorYang, Yongping; Yue, Yanling
Affiliation1.Yunnan Agr Univ, Coll Landscape & Hort, Kunming 650201, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China
3.Univ Chinese Acad Sci, Beijing, Peoples R China
4.Chinese Acad Sci, Kunming Inst Zool, Kunming, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Pu, Yanan,Hou, Lingyun,Guo, Yingqi,et al. Genome-wide analysis of the callose enzyme families of fertile and sterile flower buds of the Chinese cabbage (Brassica rapa L. ssp. pekinensis)[J]. FEBS OPEN BIO,2019,9(8):1432-1449.
APA Pu, Yanan,Hou, Lingyun,Guo, Yingqi,Ullah, Ikram,Yang, Yongping,&Yue, Yanling.(2019).Genome-wide analysis of the callose enzyme families of fertile and sterile flower buds of the Chinese cabbage (Brassica rapa L. ssp. pekinensis).FEBS OPEN BIO,9(8),1432-1449.
MLA Pu, Yanan,et al."Genome-wide analysis of the callose enzyme families of fertile and sterile flower buds of the Chinese cabbage (Brassica rapa L. ssp. pekinensis)".FEBS OPEN BIO 9.8(2019):1432-1449.
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