| Recombinant polyphenol oxidases for production of theaflavins from tea polyphenols | |
Zeng, Jun1,2; Du, Gang1; Shao, Xue1,2; Feng, Ke-Na1,2 ; Zeng, Ying1
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| 通讯作者 | Zeng, Ying(biochem@mail.kib.ac.cn) |
| 2019-08-01 | |
| 发表期刊 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
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| ISSN | 0141-8130 |
| 卷号 | 134页码:139-145 |
| 摘要 | Theaflavins (TFs) have attracted much attention due to their various bioactivities in black tea. This paper describes the first trial for enzymatic production of TFs by recombinant polyphenol oxidases (PPOs). PPO genes were cloned from nine species and expressed in E. coli. Crude enzyme assays by LC-MS revealed that eight recombinant PPOs were active for TFs production from tea polyphenols as substrates. Much higher activities were observed for crude enzymes of Md2 from Malus domestica (apple), Pp4 from Pyrus pashia (pear), and Ej2 from Eriobotrya japonica (loquat). When immobilized on mesoporous silica, crude Md2 was most active. The purified Md2 was immobilized and showed almost twice activity as high as its free enzyme. While the maximum activity of free enzyme was found at pH 5 and 10-30 degrees C, the immobilized enzyme had broader range of pH 4-6 and 10-40 degrees C. The activity of immobilized enzyme was relatively constant during the pH and thermal stability test. When used at 0.2 mg/ml in the beginning, the immobilized enzyme retained approximately 40% of its initial activity after 8 cycles of operation. (C) 2019 Elsevier B.V. All rights reserved. |
| 关键词 | Theaflavins Recombinant polyphenol oxidase Mesoporous silica |
| DOI | 10.1016/j.ijbiomac.2019.04.142 |
| 收录类别 | SCI |
| 语种 | 英语 |
| WOS记录号 | WOS:000474673600016 |
| 引用统计 | |
| 文献类型 | 期刊论文 |
| 条目标识符 | http://ir.kib.ac.cn/handle/151853/67841 |
| 专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
| 通讯作者 | Zeng, Ying |
| 作者单位 | 1.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Nat Med Chem, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zeng, Jun,Du, Gang,Shao, Xue,et al. Recombinant polyphenol oxidases for production of theaflavins from tea polyphenols[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2019,134:139-145. |
| APA | Zeng, Jun,Du, Gang,Shao, Xue,Feng, Ke-Na,&Zeng, Ying.(2019).Recombinant polyphenol oxidases for production of theaflavins from tea polyphenols.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,134,139-145. |
| MLA | Zeng, Jun,et al."Recombinant polyphenol oxidases for production of theaflavins from tea polyphenols".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 134(2019):139-145. |
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| Zeng-2019-Recombinan（1259KB） | 期刊论文 | 出版稿 | 开放获取 | CC BY-NC-SA | 浏览 下载 | |
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