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Genome sequence of Malania oleifera, a tree with great value for nervonic acid production
Xu, Chao-Qun1; Liu, Hui1; Zhou, Shan-Shan1; Zhang, Dong-Xu2; Zhao, Wei1; Wang, Sihai3; Chen, Fu4; Sun, Yan-Qiang1; Nie, Shuai1; Jia, Kai-Hua1; Jiao, Si-Qian1; Zhang, Ren-Gang5; Yun, Quan-Zheng5; Guan, Wenbin1; Wang, Xuewen4,6; Gao, Qiong1; Bennetzen, Jeffrey L.4,6; Maghuly, Fatemeh7; Porth, Ilga8,9,10; Van de Peer, Yves11,12,13; Wang, Xiao-Ru1,14; Ma, Yongpeng15; Mao, Jian-Feng1
Corresponding AuthorMa, Yongpeng( ; Mao, Jian-Feng(
Source PublicationGIGASCIENCE
AbstractBackground Malania oleifera, a member of the Olacaceae family, is an IUCN red listed tree, endemic and restricted to the Karst region of southwest China. This tree's seed is valued for its high content of precious fatty acids (especially nervonic acid). However, studies on its genetic makeup and fatty acid biogenesis are severely hampered by a lack of molecular and genetic tools. Findings We generated 51 Gb and 135Gb of raw DNA sequences, using Pacific Biosciences (PacBio) single-molecule real-time and 10x Genomics sequencing, respectively. A final genome assembly, with a scaffold N50 size of 4.65 Mb and a total length of 1.51Gb, was obtained by primary assembly based on PacBio long reads plus scaffolding with 10x Genomics reads. Identified repeats constituted approximate to 82% of the genome, and 24,064 protein-coding genes were predicted with high support. The genome has low heterozygosity and shows no evidence for recent whole genome duplication. Metabolic pathway genes relating to the accumulation of long-chain fatty acid were identified and studied in detail. Conclusions Here, we provide the first genome assembly and gene annotation for M. oleifera. The availability of these resources will be of great importance for conservation biology and for the functional genomics of nervonic acid biosynthesis.
Keywordde novo genome assembly vulnerable plant Malania nervonic acid transcriptomes
Indexed BySCI
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000462551600008
Citation statistics
Document Type期刊论文
Corresponding AuthorMa, Yongpeng; Mao, Jian-Feng
Affiliation1.Beijing Forestry Univ, Sch Nat Conservat, Beijing Adv Innovat Ctr Tree Breeding Mol Design, Natl Engn Lab Tree Breeding,Coll Biol Sci & Techn, Beijing 100083, Peoples R China
2.Datong Univ, Coll Life Sci, Datong 037009, Shanxi, Peoples R China
3.Yunnan Acad Forestry, State Forestry Adm Key Lab Yunnan Rare & Endanger, Yunnan Key Lab Forest Plant Cultivat & Utilizat, Kunming 650201, Yunnan, Peoples R China
4.Yunnan Acad Forestry, Camellia Inst, Guangnan 663300, Yunnan, Peoples R China
5.Beijing Ori Gene Sci & Technol Co Ltd, Beijing 102206, Peoples R China
6.Univ Georgia, Dept Genet, Athens, GA 30602 USA
7.Univ Nat Resources & Life Sci, PBU, Dept Biotechnol, BOKU VIBT, Muthgasse 18, A-1190 Vienna, Austria
8.Univ Laval, Dept Sci Bois & Foret, 1030 Ave Med, Quebec City, PQ G1V 0A6, Canada
9.Univ Laval, Inst Syst & Integrated Biol, Pavillon Charles Eugene Marchand,1030 Ave Med, Quebec City, PQ G1V 0A6, Canada
10.Univ Laval, Ctr Etud Foret, 1030 Ave Med, Quebec City, PQ G1V 0A6, Canada
11.Univ Ghent, Dept Plant Biotechnol & Bioinformat, B-9052 Ghent, Belgium
12.VIB Ctr Plant Syst Biol, B-9052 Ghent, Belgium
13.Univ Pretoria, Dept Biochem Genet & Microbiol Genet, Ctr Microbial Ecol & Genom, Private Bag X20, ZA-0028 Pretoria, South Africa
14.Umea Univ, UPSC, Dept Ecol & Environm Sci, SE-90187 Umea, Sweden
15.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Integrat Conservat Plant Species E, Kunming 650201, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Xu, Chao-Qun,Liu, Hui,Zhou, Shan-Shan,et al. Genome sequence of Malania oleifera, a tree with great value for nervonic acid production[J]. GIGASCIENCE,2019,8(2):14.
APA Xu, Chao-Qun.,Liu, Hui.,Zhou, Shan-Shan.,Zhang, Dong-Xu.,Zhao, Wei.,...&Mao, Jian-Feng.(2019).Genome sequence of Malania oleifera, a tree with great value for nervonic acid production.GIGASCIENCE,8(2),14.
MLA Xu, Chao-Qun,et al."Genome sequence of Malania oleifera, a tree with great value for nervonic acid production".GIGASCIENCE 8.2(2019):14.
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