The Effects of Age and Reproduction on the Lipidome of Caenorhabditis elegans
Wan, Qin-Li1; Yang, Zhong-Lin2,3; Zhou, Xiao-Gang1; Ding, Ai-Jun1; Pu, Yuan-Zhu1; Luo, Huai-Rong1,2; Wu, Gui-Sheng1
Corresponding AuthorWu, Gui-Sheng(
AbstractAging is a complex life process, and a unified view is that metabolism plays key roles in all biological processes. Here, we determined the lipidomic profile of Caenorhabditis elegans (C. elegans) using ultraperformance liquid chromatography high-resolution mass spectrometry (UPLC-HRMS). Using a nontargeted approach, we detected approximately 3000 species. Analysis of the lipid metabolic profiles at young adult and ten-day-old ages among wild-type N2, glp-1 defective mutant, and double mutant daf-16;glp-1 uncovered significant age-related differences in the total amount of phosphatidylcholines (PC), sphingomyelins (SM), ceramides (Cer), diglycerides (DG), and triglycerides (TG). In addition, the age-associated lipid profiles were characterized by ratio of polyunsaturated (PUFA) over monounsaturated (MUFA) lipid species. Lipid metabolism modulation plays an important role in reproduction-regulated aging; to identify the variations of lipid metabolites during germ line loss-induced longevity, we investigated the lipidomic profiles of long-lived glp-1/notch receptor mutants, which have reproductive deficiency when grown at nonpermissive temperature. The results showed that there was some age-related lipid variation, including TG 40:2, TG 40:1, and TG 41:1, which contributed to the long-life phenotype. The longevity of glp-1 mutant was daf-16-dependent; the lipidome analysis of daf-16;glp-1 double mutant revealed that the changes of some metabolites in the glp-1 mutant were daf-16-dependent, while other metabolites displayed more complex epistatic patterns. We first conducted a comprehensive lipidome analysis to provide novel insights into the relationships between longevity and lipid metabolism regulated by germ line signals in C. elegans.
Indexed BySCI
WOS Research AreaCell Biology
WOS SubjectCell Biology
WOS IDWOS:000468566800001
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Document Type期刊论文
Corresponding AuthorWu, Gui-Sheng
Affiliation1.Southwest Med Univ, Sch Pharm, Dept Pharmacol, Key Lab Aging & Regenerat Med, Luzhou 646000, Sichuan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Yunnan Key Lab Nat Med Chem, Kunming 650201, Yunnan, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100039, Peoples R China
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Wan, Qin-Li,Yang, Zhong-Lin,Zhou, Xiao-Gang,et al. The Effects of Age and Reproduction on the Lipidome of Caenorhabditis elegans[J]. OXIDATIVE MEDICINE AND CELLULAR LONGEVITY,2019:14.
APA Wan, Qin-Li.,Yang, Zhong-Lin.,Zhou, Xiao-Gang.,Ding, Ai-Jun.,Pu, Yuan-Zhu.,...&Wu, Gui-Sheng.(2019).The Effects of Age and Reproduction on the Lipidome of Caenorhabditis elegans.OXIDATIVE MEDICINE AND CELLULAR LONGEVITY,14.
MLA Wan, Qin-Li,et al."The Effects of Age and Reproduction on the Lipidome of Caenorhabditis elegans".OXIDATIVE MEDICINE AND CELLULAR LONGEVITY (2019):14.
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