Characterization of a sesquiterpene cyclase from the glandular trichomes of Leucosceptrum canum for sole production of cedrol in Escherichia coli and Nicotiana benthamiana

doi:10.1016/j.phytochem.2019.03.009

	Characterization of a sesquiterpene cyclase from the glandular trichomes of Leucosceptrum canum for sole production of cedrol in Escherichia coli and Nicotiana benthamiana
	Luo, Fei 1,2,3; Ling, Yi 1,2; Li, De-Sen 1,2; Tang, Ting 1,2; Liu, Yan-Chun1,2,3 ; Liu, Yan 1,2; Li, Sheng-Hong1,2
通讯作者	Liu, Yan(liuyan@mail.kib.ac.cn) ; Li, Sheng-Hong(shli@mail.kib.ac.cn)
	2019-06-01
发表期刊	PHYTOCHEMISTRY
ISSN	0031-9422
卷号	162 页码:121-128
摘要	Cedrol is an extremely versatile sesquiterpene alcohol that was approved by the Food and Drug Administration of the United States as a flavoring agent or adjuvant and has been commonly used as a flavoring ingredient in cosmetics, foods and medicine. Furthermore, cedrol possesses a wide range of pharmacological properties including sedative, anti-inflammatory and cytotoxic activities. Commercial production of cedrol relies on fractional distillation of cedar wood oils, followed by recrystallization, and little has been reported about its biosynthesis and aspects of synthetic biology. Here, we report the cloning and functional characterization of a cedrol synthase gene (Lc-CedS) from the transcriptome of the glandular trichomes of a woody Lamiaceae plant Leucosceptrum canton. The recombinant Lc-CedS protein catalyzed the in vitro conversion of farnesyl diphosphate into the single product cedrol, suggesting that Lc-CedS is a high-fidelity terpene synthase. Co-expression of Lc-CedS, a farnesyl diphosphate synthase gene and seven genes of the mevalonate (MVA) pathway responsible for converting acetyl-CoA into farnesyl diphosphate in Escherichia coli afforded 363 mu g/L cedrol as the sole product under shaking flask conditions. Transient expression of Lc-CedS in Nicotiana benthamiana also resulted in a single product cedrol with a production level of 3.6 mu g/g fresh weight. The sole production of cedrol by introducing of Lc-CedS in engineered E. colt and N. benthamiana suggests now alternative production systems using synthetic biology approaches that would better address sufficient supply of cedrol.
关键词	Leucosceptrum canum Lamiaceae Cedrol Sesquiterpene cylase Cedrol synthase Heterologous production Synthetic biology
DOI	10.1016/j.phytochem.2019.03.009
收录类别	SCI
语种	英语
WOS记录号	WOS:000469157200011
引用统计
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/67399
专题	植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Liu, Yan; Li, Sheng-Hong
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 2.Yunnan Key Lab Nat Med Chem, Kunming 650201, Yunnan, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Luo, Fei,Ling, Yi,Li, De-Sen,et al. Characterization of a sesquiterpene cyclase from the glandular trichomes of Leucosceptrum canum for sole production of cedrol in Escherichia coli and Nicotiana benthamiana[J]. PHYTOCHEMISTRY,2019,162:121-128.
APA	Luo, Fei.,Ling, Yi.,Li, De-Sen.,Tang, Ting.,Liu, Yan-Chun.,...&Li, Sheng-Hong.(2019).Characterization of a sesquiterpene cyclase from the glandular trichomes of Leucosceptrum canum for sole production of cedrol in Escherichia coli and Nicotiana benthamiana.PHYTOCHEMISTRY,162,121-128.
MLA	Luo, Fei,et al."Characterization of a sesquiterpene cyclase from the glandular trichomes of Leucosceptrum canum for sole production of cedrol in Escherichia coli and Nicotiana benthamiana".PHYTOCHEMISTRY 162(2019):121-128.

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