Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, "Gao-ben") by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure

doi:10.3389/fpls.2019.00429

	Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, "Gao-ben") by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure
	Liu, Zhen-wen 1; Gao, Yu-zhen 2,3; Zhou, Jing 2,3
通讯作者	Zhou, Jing(zhoujing_apiaceae@163.com)
	2019-04-09
发表期刊	FRONTIERS IN PLANT SCIENCE
ISSN	1664-462X
卷号	10 页码:8
摘要	Ligustici Rhizoma et Radix (LReR), an important Chinese medicine known as "Gaoben," refers to Ligusticum sinense Oliv. or Ligusticum jeholense Nakai et Kitag. However, a number of other species are commonly sold as "Gao-ben" in the herbal medicine market, which may result in a series of quality control problems and inconsistent therapeutic effects. The "Gao-ben" is commonly sold sliced and dried, making traditional identification methods difficult. Here, the mini barcode ITS2 region was examined on 68 samples representing LReR and 7 potential adulterant or substitute species. The results showed 100% success rates of PCR and sequencing and the existence of a barcoding gap. The neighbor-joining (NJ) tree indicated that all the tested samples could be exactly identified. The ITS2 secondary structure revealed a clear difference between true "Gaoben" and three adulterant species. We therefore recommend the use of ITS2 as a mini barcode for distinguishing between closely or distantly related plant species that may be used in Chinese medicine.
关键词	apiaceae DNA barcoding Ligustici Rhizoma et Radix ITS2 secondary structures
DOI	10.3389/fpls.2019.00429
收录类别	SCI
语种	英语
WOS记录号	WOS:000463915400001
引用统计	被引频次：22[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/66165
专题	中国科学院东亚植物多样性与生物地理学重点实验室
通讯作者	Zhou, Jing
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, CAS Key Lab Plant Divers & Biogeog East Asia, Kunming, Yunnan, Peoples R China 2.Kunming Med Univ, Sch Pharmaceut Sci, Kunming, Yunnan, Peoples R China 3.Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Liu, Zhen-wen,Gao, Yu-zhen,Zhou, Jing. Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, "Gao-ben") by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure[J]. FRONTIERS IN PLANT SCIENCE,2019,10:8.
APA	Liu, Zhen-wen,Gao, Yu-zhen,&Zhou, Jing.(2019).Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, "Gao-ben") by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure.FRONTIERS IN PLANT SCIENCE,10,8.
MLA	Liu, Zhen-wen,et al."Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, "Gao-ben") by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure".FRONTIERS IN PLANT SCIENCE 10(2019):8.

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