Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, "Gao-ben") by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure
Liu, Zhen-wen1; Gao, Yu-zhen2,3; Zhou, Jing2,3
Corresponding AuthorZhou, Jing(zhoujing_apiaceae@163.com)
2019-04-09
Source PublicationFRONTIERS IN PLANT SCIENCE
ISSN1664-462X
Volume10Pages:8
AbstractLigustici Rhizoma et Radix (LReR), an important Chinese medicine known as "Gaoben," refers to Ligusticum sinense Oliv. or Ligusticum jeholense Nakai et Kitag. However, a number of other species are commonly sold as "Gao-ben" in the herbal medicine market, which may result in a series of quality control problems and inconsistent therapeutic effects. The "Gao-ben" is commonly sold sliced and dried, making traditional identification methods difficult. Here, the mini barcode ITS2 region was examined on 68 samples representing LReR and 7 potential adulterant or substitute species. The results showed 100% success rates of PCR and sequencing and the existence of a barcoding gap. The neighbor-joining (NJ) tree indicated that all the tested samples could be exactly identified. The ITS2 secondary structure revealed a clear difference between true "Gaoben" and three adulterant species. We therefore recommend the use of ITS2 as a mini barcode for distinguishing between closely or distantly related plant species that may be used in Chinese medicine.
Keywordapiaceae DNA barcoding Ligustici Rhizoma et Radix ITS2 secondary structures
DOI10.3389/fpls.2019.00429
Indexed BySCI
Language英语
WOS Research AreaPlant Sciences
WOS SubjectPlant Sciences
WOS IDWOS:000463915400001
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/66165
Collection中国科学院东亚植物多样性与生物地理学重点实验室
Corresponding AuthorZhou, Jing
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, CAS Key Lab Plant Divers & Biogeog East Asia, Kunming, Yunnan, Peoples R China
2.Kunming Med Univ, Sch Pharmaceut Sci, Kunming, Yunnan, Peoples R China
3.Kunming Med Univ, Yunnan Key Lab Pharmacol Nat Prod, Kunming, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Liu, Zhen-wen,Gao, Yu-zhen,Zhou, Jing. Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, "Gao-ben") by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure[J]. FRONTIERS IN PLANT SCIENCE,2019,10:8.
APA Liu, Zhen-wen,Gao, Yu-zhen,&Zhou, Jing.(2019).Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, "Gao-ben") by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure.FRONTIERS IN PLANT SCIENCE,10,8.
MLA Liu, Zhen-wen,et al."Molecular Authentication of the Medicinal Species of Ligusticum (Ligustici Rhizoma et Radix, "Gao-ben") by Integrating Non-coding Internal Transcribed Spacer 2 (ITS2) and Its Secondary Structure".FRONTIERS IN PLANT SCIENCE 10(2019):8.
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