Characterization of 24 microsatellite markers in Primula chungensis (Primulaceae), a distylous-homostylous species, using MiSeq sequencing. | |
Zhou, Wei; Li, Haidong; Wu, Zhikun; Barrett, Spencer C H; Li, Dezhu; Wang, Hong | |
2016 | |
发表期刊 | Plant diversity |
ISSN | 2468-2659 |
卷号 | 38期号:2页码:89-91 |
摘要 | Primula chungensis is a species with considerable floral and mating-system variation, including distylous (outcrossing), homostylous (selfing) and mixed populations that contain both outcrossing and selfing forms. We isolated 24 microsatellite markers from P. chungensis using Illumina MiSeq sequencing. Polymorphism and genetic diversity were then measured based on a sample of 24 individuals from a natural population in southern Tibet. All loci were polymorphic with the number of alleles per locus ranging from 2 to 4. The observed and expected heterozygosity ranged from 0 to 1 and 0.219 to 0.708, respectively. The microsatellite markers we have identified will serve as valuable tools for the investigation of the population genetic structure and phylogeography of P. chungensis and will inform models of the evolutionary history of mating systems in the species. |
文献类型 | 期刊论文 |
条目标识符 | http://ir.kib.ac.cn/handle/151853/64897 |
专题 | 植物化学与西部植物资源持续利用国家重点实验室 |
推荐引用方式 GB/T 7714 | Zhou, Wei,Li, Haidong,Wu, Zhikun,et al. Characterization of 24 microsatellite markers in Primula chungensis (Primulaceae), a distylous-homostylous species, using MiSeq sequencing.[J]. Plant diversity,2016,38(2):89-91. |
APA | Zhou, Wei,Li, Haidong,Wu, Zhikun,Barrett, Spencer C H,Li, Dezhu,&Wang, Hong.(2016).Characterization of 24 microsatellite markers in Primula chungensis (Primulaceae), a distylous-homostylous species, using MiSeq sequencing..Plant diversity,38(2),89-91. |
MLA | Zhou, Wei,et al."Characterization of 24 microsatellite markers in Primula chungensis (Primulaceae), a distylous-homostylous species, using MiSeq sequencing.".Plant diversity 38.2(2016):89-91. |
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