Identification and Characterization of a delta-Cadinol Synthase Potentially Involved in the Formation of Boreovibrins in Boreostereum vibrans of Basidiomycota.

	Identification and Characterization of a delta-Cadinol Synthase Potentially Involved in the Formation of Boreovibrins in Boreostereum vibrans of Basidiomycota.
	Zhou, Hui; Yang, Yan-Long; Zeng, Jun; Zhang, Ling; Ding, Zhi-Hui; Zeng, Ying
	2016
发表期刊	Natural products and bioprospecting
ISSN	2192-2195
卷号	6 期号:3 页码:167-71
摘要	Sesquiterpenoids are very common among natural products. A large number of sesquiterpene synthase genes have been cloned and functionally characterized. However, until now there is no report about the delta-cadinol synthase predominantly forming delta-cadinol (syn. torreyol) from farnesyl diphosphate. Sesquiterpenoids boreovibrins structurally similar to delta-cadinol were previously isolated from culture broths of the basidiomycete fungus Boreostereum vibrans. This led us to expect a corresponding gene coding for a delta-cadinol synthase that may be involved in the biosynthesis of boreovibrins in B. vibrans. Here we report the cloning and heterologous expression of a new sesquiterpene synthase gene from B. vibrans. The crude and purified recombinant enzymes, when incubating with farnesyl diphosphate as substrate, gave delta-cadinol as its principal product and thereby identified as a delta-cadinol synthase. A new sesquiterpene synthase gene was cloned from the basidiomycete fungus Boreostereum vibrans and heterologously expressed in E. coli. The purified recombinant enzyme gave delta-cadinol as its principal product from farnesyl diphosphate and thereby identified as a delta-cadinol synthase (BvCS).
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/64888
专题	植物化学与西部植物资源持续利用国家重点实验室
推荐引用方式 GB/T 7714	Zhou, Hui,Yang, Yan-Long,Zeng, Jun,et al. Identification and Characterization of a delta-Cadinol Synthase Potentially Involved in the Formation of Boreovibrins in Boreostereum vibrans of Basidiomycota.[J]. Natural products and bioprospecting,2016,6(3):167-71.
APA	Zhou, Hui,Yang, Yan-Long,Zeng, Jun,Zhang, Ling,Ding, Zhi-Hui,&Zeng, Ying.(2016).Identification and Characterization of a delta-Cadinol Synthase Potentially Involved in the Formation of Boreovibrins in Boreostereum vibrans of Basidiomycota..Natural products and bioprospecting,6(3),167-71.
MLA	Zhou, Hui,et al."Identification and Characterization of a delta-Cadinol Synthase Potentially Involved in the Formation of Boreovibrins in Boreostereum vibrans of Basidiomycota.".Natural products and bioprospecting 6.3(2016):167-71.