Molecular cloning and expression analysis of turnip (Brassica rapa var. rapa) sucrose transporter gene family.
Liu, Yuanyuan; Yin, Xin; Yang, Ya; Wang, Chuntao; Yang, Yongping
2017
发表期刊Plant diversity
ISSN2468-2659
卷号39期号:3页码:123-129
摘要In higher plants, sugars (mainly sucrose) are produced by photosynthetically assimilated carbon in mesophyll cells of leaves and translocated to heterotrophic organs to ensure plant growth and development. Sucrose transporters, or sucrose carriers (SUCs), play an important role in the long-distance transportation of sucrose from source organs to sink organs, thereby affecting crop yield and quality. The identification, characterization, and molecular function analysis of sucrose transporter genes have been reported for monocot and dicot plants. However, no relevant study has been reported on sucrose transporter genes in Brassica rapa var. rapa, a cruciferous root crop used mainly as vegetables and fodder. We identified and cloned 12 sucrose transporter genes from turnips, named BrrSUC1.1 to BrrSUC6.2 according to the SUC gene sequences of B.rapa pekinensis. We constructed a phylogenetic tree and analyzed conserved motifs for all 12 sucrose transporter genes identified. Real-time quantitative polymerase chain reaction was conducted to understand the expression levels of SUC genes in different tissues and developmental phases of the turnip. These findings add to our understanding of the genetics and physiology of sugar transport during taproot formation in turnips.
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/64846
专题植物化学与西部植物资源持续利用国家重点实验室
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GB/T 7714
Liu, Yuanyuan,Yin, Xin,Yang, Ya,et al. Molecular cloning and expression analysis of turnip (Brassica rapa var. rapa) sucrose transporter gene family.[J]. Plant diversity,2017,39(3):123-129.
APA Liu, Yuanyuan,Yin, Xin,Yang, Ya,Wang, Chuntao,&Yang, Yongping.(2017).Molecular cloning and expression analysis of turnip (Brassica rapa var. rapa) sucrose transporter gene family..Plant diversity,39(3),123-129.
MLA Liu, Yuanyuan,et al."Molecular cloning and expression analysis of turnip (Brassica rapa var. rapa) sucrose transporter gene family.".Plant diversity 39.3(2017):123-129.
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