Mechanistic insights into GLUT1 activation and clustering revealed by super-resolution imaging

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	Mechanistic insights into GLUT1 activation and clustering revealed by super-resolution imaging
	Yan, Qiuyan; Lu, Yanting; Zhou, Lulu; Chen, Junling; Xu, Haijiao; Cai, Mingjun; Shi, Yan; Jiang, Junguang; Xiong, Wenyong; Gao, Jing; Wang, Hongda
	2018
发表期刊	PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
ISSN	0027-8424
卷号	115 期号:27 页码:7033-7038
摘要	The glucose transporter GLUT1, a plasma membrane protein that mediates glucose homeostasis in mammalian cells, is responsible for constitutive uptake of glucose into many tissues and organs. Many studies have focused on its vital physiological functions and close relationship with diseases. However, the molecular mechanisms of its activation and transport are not clear, and its detailed distribution pattern on cell membranes also remains unknown. To address these, we first investigated the distribution and assembly of GLUT1 at a nanometer resolution by super-resolution imaging. On HeLa cell membranes, the transporter formed clusters with an average diameter of similar to 250 nm, the majority of which were regulated by lipid rafts, as well as being restricted in size by both the cytoskeleton and glycosylation. More importantly, we found that the activation of GLUT1 by azide or M beta CD did not increase its membrane expression but induced the decrease of the large clusters. The results suggested that sporadic distribution of GLUT1 may facilitate the transport of glucose, implying a potential association between the distribution and activation. Collectively, our work characterized the clustering distribution of GLUT1 and linked its spatial structural organization to the functions, which would provide insights into the activation mechanism of the transporter.
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/64712
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Yan, Qiuyan,Lu, Yanting,Zhou, Lulu,et al. Mechanistic insights into GLUT1 activation and clustering revealed by super-resolution imaging[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2018,115(27):7033-7038.
APA	Yan, Qiuyan.,Lu, Yanting.,Zhou, Lulu.,Chen, Junling.,Xu, Haijiao.,...&Wang, Hongda.(2018).Mechanistic insights into GLUT1 activation and clustering revealed by super-resolution imaging.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,115(27),7033-7038.
MLA	Yan, Qiuyan,et al."Mechanistic insights into GLUT1 activation and clustering revealed by super-resolution imaging".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 115.27(2018):7033-7038.