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Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens
Wang, Yi; Geng, Changan; Yuan, Xiaolong; Hua, Mei; Tian, Fenghua; Li, Changtian
Corresponding AuthorLi, Changtian(lct@jlau.edu.cn)
2018
Source PublicationPLOS ONE
ISSN1932-6203
Volume13Issue:7Pages:e0199110
AbstractUsnic acid is a unique polyketide produced by lichens. To characterize usnic acid biosynthesis, the transcriptome of the usnic-acid-producing lichen-forming fungus Nephromopsis pallescens was sequenced using Illumina NextSeq technology. Seven complete non-reducing polyketide synthase genes and nine highly-reducing polyketide synthase genes were obtained through transcriptome analysis. Gene expression results obtained by qPCR and usnic acid detection with LCMS-IT-TOF showed that Nppks7 is probably involved in usnic acid biosynthesis in N. pallescens. Nppks7 is a non-reducing polyketide synthase with a MeT domain that also possesses beta-ketoacyl-ACP synthase, acyl transferase, product template, acyl carrier protein, C-methyltransferase, and Claisen cyclase domains. Phylogenetic analysis shows that Nppks7 and other polyketide synthases from lichens form a unique monophyletic Glade. Taken together, our data indicate that Nppks7 is a novel PKS in N. pallescens that is likely involved in usnic acid biosynthesis.
DOI10.1371/journal.pone.0199110
Indexed BySCI
Language英语
WOS Research AreaScience & Technology - Other Topics
WOS SubjectMultidisciplinary Sciences
WOS IDWOS:000439022400009
Citation statistics
Cited Times:5[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/64708
Collection中国科学院昆明植物研究所
Recommended Citation
GB/T 7714
Wang, Yi,Geng, Changan,Yuan, Xiaolong,et al. Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens[J]. PLOS ONE,2018,13(7):e0199110.
APA Wang, Yi,Geng, Changan,Yuan, Xiaolong,Hua, Mei,Tian, Fenghua,&Li, Changtian.(2018).Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens.PLOS ONE,13(7),e0199110.
MLA Wang, Yi,et al."Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens".PLOS ONE 13.7(2018):e0199110.
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