Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens

doi:10.1371/journal.pone.0199110

KIB OpenIR

	Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens
	Wang, Yi; Geng, Changan; Yuan, Xiaolong; Hua, Mei; Tian, Fenghua; Li, Changtian
通讯作者	Li, Changtian(lct@jlau.edu.cn)
	2018
发表期刊	PLOS ONE
ISSN	1932-6203
卷号	13 期号:7 页码:e0199110
摘要	Usnic acid is a unique polyketide produced by lichens. To characterize usnic acid biosynthesis, the transcriptome of the usnic-acid-producing lichen-forming fungus Nephromopsis pallescens was sequenced using Illumina NextSeq technology. Seven complete non-reducing polyketide synthase genes and nine highly-reducing polyketide synthase genes were obtained through transcriptome analysis. Gene expression results obtained by qPCR and usnic acid detection with LCMS-IT-TOF showed that Nppks7 is probably involved in usnic acid biosynthesis in N. pallescens. Nppks7 is a non-reducing polyketide synthase with a MeT domain that also possesses beta-ketoacyl-ACP synthase, acyl transferase, product template, acyl carrier protein, C-methyltransferase, and Claisen cyclase domains. Phylogenetic analysis shows that Nppks7 and other polyketide synthases from lichens form a unique monophyletic Glade. Taken together, our data indicate that Nppks7 is a novel PKS in N. pallescens that is likely involved in usnic acid biosynthesis.
DOI	10.1371/journal.pone.0199110
收录类别	SCI
语种	英语
WOS记录号	WOS:000439022400009
引用统计	被引频次：24[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/64708
专题	中国科学院昆明植物研究所
推荐引用方式 GB/T 7714	Wang, Yi,Geng, Changan,Yuan, Xiaolong,et al. Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens[J]. PLOS ONE,2018,13(7):e0199110.
APA	Wang, Yi,Geng, Changan,Yuan, Xiaolong,Hua, Mei,Tian, Fenghua,&Li, Changtian.(2018).Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens.PLOS ONE,13(7),e0199110.
MLA	Wang, Yi,et al."Identification of a putative polyketide synthase gene involved in usnic acid biosynthesis in the lichen Nephromopsis pallescens".PLOS ONE 13.7(2018):e0199110.