Diterpenoid compounds from Wedelia trilobata induce resistance to Tomato spotted wilt virus via the JA signal pathway in tobacco plants
Zhao, Lihua1; Hu, Zhonghui2; Li, Shunlin2; Zhou, Xueping1,3; Li, Jing4; Su, Xiaoxia1; Zhang, Lizhen1; Zhang, Zhongkai1; Dong, Jiahong1
2019-02-26
Source PublicationSCIENTIFIC REPORTS
ISSN2045-2322
Volume9Pages:12
AbstractTomato spotted wilt virus (TSWV) causes major losses of many crops worldwide. Several strategies have been attempted to control disease caused by TSWV. However, many challenges for the effective control of this disease remain. A promising approach is the use of abiotic or biotic inducers to enhance plant resistance to pathogens. We screened a diterpenoid compound from Wedelia trilobata, 3 alpha-Angeloyloxy-9 beta-hydroxy-ent-kaur-16-en-19-oic acid (AHK), which had higher curative and protective effects against TSWV than the ningnanmycin control. The rapid initiation of the expression of all the TSWV genes was delayed by more than 1d in the curative assay, and the expression of the NSs, NSm and RdRp genes was inhibited. In addition, the replication of all TSWV genes in systemic leaves was inhibited in the protective assay, with an inhibition rate of more than 90%. The concentrations of jasmonic acid (JA) and jasmonic acid isoleucine (JA-ILE) in the AHK-treated and systemic leaves of the treated plants were significantly higher than those observed in the control. The results suggested that AHK can induce systemic resistance in treated plants. The transcription of the NtCOI1 gene, a key gene in the JA pathway, was significantly higher in both the inoculated and systemic leaves of the AHK-treated plants compared to the control. The AHK-induced resistance to TSWV in Nicotiana benthamiana could be eliminated by VIGS-mediated silencing of the NtCOI1 gene. These results indicated that AHK can activate the JA pathway and induce systemic resistance to TSWV infection.
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/64627
Collection植物化学与西部植物资源持续利用国家重点实验室
Corresponding AuthorZhang, Zhongkai; Dong, Jiahong
Affiliation1.Minist Agr, Inst Biotechnol & Germplasm Resources, Key Lab Southwestern Crop Gene Resource & Germpla, Yunnan Acad Agr Sci,Yunnan Prov Key Lab Agr Biote, Kunming 650204, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China
3.Zhejiang Univ, Inst Biotechnol, Hangzhou 310058, Zhejiang, Peoples R China
4.Southwest Forestry Univ, Life Sci Coll, Kunming 650224, Yunnan, Peoples R China
Recommended Citation
GB/T 7714
Zhao, Lihua,Hu, Zhonghui,Li, Shunlin,et al. Diterpenoid compounds from Wedelia trilobata induce resistance to Tomato spotted wilt virus via the JA signal pathway in tobacco plants[J]. SCIENTIFIC REPORTS,2019,9:12.
APA Zhao, Lihua.,Hu, Zhonghui.,Li, Shunlin.,Zhou, Xueping.,Li, Jing.,...&Dong, Jiahong.(2019).Diterpenoid compounds from Wedelia trilobata induce resistance to Tomato spotted wilt virus via the JA signal pathway in tobacco plants.SCIENTIFIC REPORTS,9,12.
MLA Zhao, Lihua,et al."Diterpenoid compounds from Wedelia trilobata induce resistance to Tomato spotted wilt virus via the JA signal pathway in tobacco plants".SCIENTIFIC REPORTS 9(2019):12.
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