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拟南芥光形态突变体的筛选与基因鉴定
郭涛 ; 李婉莎 ; 查向东 ; 胡向阳
2011
Source Publication植物分类与资源学报
Issue6Pages:645-652
Abstract以哥伦比亚(Columbia)野生型拟南芥(Arabidopsis thaliana)为实验材料,用含有激活标记双元质粒pCB260的农杆菌浸花进行转化,构建拟南芥T-DNA插入突变体库。通过突变体的筛选和表型分析,获得了两株光形态突变体,子叶下胚轴伸长的光抑制效应减弱。通过TAIL-PCR(thermal asymmetric interlaced-PCR)技术,成功扩增出突变植株T-DNA插入位点侧翼序列,经NCBI序列比对,T-DNA分别插在CRY1第一和第三外显子部位。突变体的表型分析及PCR鉴定结果表明,T-DNA插入CRY1并影响到突变植株的光形态建成。
Indexed Bycscd
Language中文
CSCD IDCSCD:4393451
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/6449
Collection中国科学院青藏高原研究所昆明部
Recommended Citation
GB/T 7714
郭涛,李婉莎,查向东,等. 拟南芥光形态突变体的筛选与基因鉴定[J]. 植物分类与资源学报,2011(6):645-652.
APA 郭涛,李婉莎,查向东,&胡向阳.(2011).拟南芥光形态突变体的筛选与基因鉴定.植物分类与资源学报(6),645-652.
MLA 郭涛,et al."拟南芥光形态突变体的筛选与基因鉴定".植物分类与资源学报 .6(2011):645-652.
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