Diterpene synthases facilitating production of the kaurane skeleton of eriocalyxin B in the medicinal plant Isodon eriocalyx

	Diterpene synthases facilitating production of the kaurane skeleton of eriocalyxin B in the medicinal plant Isodon eriocalyx
	Du, Gang 1,3; Gong, Hai-Yan 1,3; Feng, Ke-Na1,3 ; Chen, Qian-Qian 1,3; Yang, Yan-Long1,3 ; Fu, Xiao-Li 1; Lu, Shan 2; Zeng, Ying1
	2019-02-01
发表期刊	PHYTOCHEMISTRY
ISSN	0031-9422
卷号	158 页码:96-102
摘要	The Isodon plants (Lamiaceae) have been used in traditional Chinese medicine to alleviate sufferings from inflammations and cancers. This feature has been attributed to the presence of pharmacologically active ent-kaurane diterpenoids such as eriocalyxin B and oridonin. The Isodon eriocalyx (Dunn) Kudo species native to southwest China can accumulate a particularly high content of ent-kaurane diterpenoids (similar to 1.5% w/w of dried leaves). We previously identified diterpene synthases IeCPS1 and IeCPS2 as ent-copalyl diphosphate synthases (ent-CPS) potentially involved in Isodon ent-kaurane diterpenoids biosynthesis. In this study, analysis of RNA-seq transcriptome of the I. eriocalyx plant revealed three other diterpene synthase genes (IeCPS3, IeKS1, and IeKSL1). Their functional characterization through coupled in vitro enzyme assays has confirmed that IeCPS3 is an ent-CPS specifically producing ent-copalyl diphosphate (ent-CPP). IeKS1 accepted ent-CPP to produce exclusively ent-kaurene and may thus be defined as an ent-kaurene synthase (ent-KS). When IeKSL1 was combined with IeCPS2 or IeCPS3, no product was detected. Based on tissue-specific expression and metabolic localization studies, the IeCPS3 and IeKS1 transcripts were significantly accumulated in leaves where the ent-kaurane diterpenoid eriocalyxin B dominates, whereas weak expression of both were observed in germinating seeds in which gibberellin biosynthetic pathway is normally active. Our findings suggest that both IeCPS3 and IeKS1 possess dual roles in general (gibberellins) and specialized diterpenoid metabolism, such as that of the Isodon ent-kaurane diterpenoids.
关键词	Isodon eriocalyx Lamiaceae Biosynthesis Diterpenoids Copalyl diphosphate synthase Kaurene synthase IeCPS3 IeKS1
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/64404
专题	植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Zeng, Ying
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Yunnan Key Lab Nat Med Chem, Kunming 650201, Yunnan, Peoples R China 2.Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210023, Jiangsu, Peoples R China 3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Du, Gang,Gong, Hai-Yan,Feng, Ke-Na,et al. Diterpene synthases facilitating production of the kaurane skeleton of eriocalyxin B in the medicinal plant Isodon eriocalyx[J]. PHYTOCHEMISTRY,2019,158:96-102.
APA	Du, Gang.,Gong, Hai-Yan.,Feng, Ke-Na.,Chen, Qian-Qian.,Yang, Yan-Long.,...&Zeng, Ying.(2019).Diterpene synthases facilitating production of the kaurane skeleton of eriocalyxin B in the medicinal plant Isodon eriocalyx.PHYTOCHEMISTRY,158,96-102.
MLA	Du, Gang,et al."Diterpene synthases facilitating production of the kaurane skeleton of eriocalyxin B in the medicinal plant Isodon eriocalyx".PHYTOCHEMISTRY 158(2019):96-102.

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