Diterpene synthases facilitating production of the kaurane skeleton of eriocalyxin B in the medicinal plant Isodon eriocalyx
Du, Gang1,3; Gong, Hai-Yan1,3; Feng, Ke-Na1,3; Chen, Qian-Qian1,3; Yang, Yan-Long1,3; Fu, Xiao-Li1; Lu, Shan2; Zeng, Ying1
2019-02-01
Source PublicationPHYTOCHEMISTRY
ISSN0031-9422
Volume158Pages:96-102
AbstractThe Isodon plants (Lamiaceae) have been used in traditional Chinese medicine to alleviate sufferings from inflammations and cancers. This feature has been attributed to the presence of pharmacologically active ent-kaurane diterpenoids such as eriocalyxin B and oridonin. The Isodon eriocalyx (Dunn) Kudo species native to southwest China can accumulate a particularly high content of ent-kaurane diterpenoids (similar to 1.5% w/w of dried leaves). We previously identified diterpene synthases IeCPS1 and IeCPS2 as ent-copalyl diphosphate synthases (ent-CPS) potentially involved in Isodon ent-kaurane diterpenoids biosynthesis. In this study, analysis of RNA-seq transcriptome of the I. eriocalyx plant revealed three other diterpene synthase genes (IeCPS3, IeKS1, and IeKSL1). Their functional characterization through coupled in vitro enzyme assays has confirmed that IeCPS3 is an ent-CPS specifically producing ent-copalyl diphosphate (ent-CPP). IeKS1 accepted ent-CPP to produce exclusively ent-kaurene and may thus be defined as an ent-kaurene synthase (ent-KS). When IeKSL1 was combined with IeCPS2 or IeCPS3, no product was detected. Based on tissue-specific expression and metabolic localization studies, the IeCPS3 and IeKS1 transcripts were significantly accumulated in leaves where the ent-kaurane diterpenoid eriocalyxin B dominates, whereas weak expression of both were observed in germinating seeds in which gibberellin biosynthetic pathway is normally active. Our findings suggest that both IeCPS3 and IeKS1 possess dual roles in general (gibberellins) and specialized diterpenoid metabolism, such as that of the Isodon ent-kaurane diterpenoids.
KeywordIsodon eriocalyx Lamiaceae Biosynthesis Diterpenoids Copalyl diphosphate synthase Kaurene synthase IeCPS3 IeKS1
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/64404
Collection植物化学与西部植物资源持续利用国家重点实验室
Corresponding AuthorZeng, Ying
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Yunnan Key Lab Nat Med Chem, Kunming 650201, Yunnan, Peoples R China
2.Nanjing Univ, Sch Life Sci, State Key Lab Pharmaceut Biotechnol, Nanjing 210023, Jiangsu, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
Recommended Citation
GB/T 7714
Du, Gang,Gong, Hai-Yan,Feng, Ke-Na,et al. Diterpene synthases facilitating production of the kaurane skeleton of eriocalyxin B in the medicinal plant Isodon eriocalyx[J]. PHYTOCHEMISTRY,2019,158:96-102.
APA Du, Gang.,Gong, Hai-Yan.,Feng, Ke-Na.,Chen, Qian-Qian.,Yang, Yan-Long.,...&Zeng, Ying.(2019).Diterpene synthases facilitating production of the kaurane skeleton of eriocalyxin B in the medicinal plant Isodon eriocalyx.PHYTOCHEMISTRY,158,96-102.
MLA Du, Gang,et al."Diterpene synthases facilitating production of the kaurane skeleton of eriocalyxin B in the medicinal plant Isodon eriocalyx".PHYTOCHEMISTRY 158(2019):96-102.
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