Bioassay-guided isolation of active compounds from Adenosma buchneroides essential oil as mosquito repellent against Aedes albopictus

	Bioassay-guided isolation of active compounds from Adenosma buchneroides essential oil as mosquito repellent against Aedes albopictus
	Ma, Yongpeng 1,2; Li, Mingxiang 1,2; Zhang, Hongxia1 ; Sun, Hongzheng 3; Su, Honghai 3; Wang, Yuhua1 ; Du, Zhizhi1
	2019-03-01
发表期刊	JOURNAL OF ETHNOPHARMACOLOGY
ISSN	0378-8741
卷号	231 页码:386-393
摘要	Ethnopharmacological relevance: A folk herb Adenosma buchneroides found in the previous ethnobotanical investigation plays an important role as an insect repellent among the Aini people in southwest of China, but the active compounds responsible for repellent activity of the plant have not yet been investigated. Aim of the study: The main purpose of the study is to identify the active components of the essential oil which responsible for its repellent activity against Aedes albopictus to support the usage of the plant as mosquito repellent by Aini people. In addition, to supply a class of potential alternatives characterized carvacrol analogues to develop natural repellent products. Material and methods: The essential oil from aerial part of Ad. buchneroides was extracted by hydrodistillation. A systematic bioassay-guided isolation of repellent compounds from the essential oil was conducted through chromatographic fractionation combined with in-cage mosquito repellent bioassay. The identification of the essential oil components was accomplished by GC-MS and GC-FID techniques. The structural elucidation of compounds was performed on the basis of IR, HR-ESI-MS and NMR. Larvicidal activity and cytotoxicity of all repellent compounds also tested by larval bioassays and MTS assays, respectively. Structure-activity relationship (SAR) of carvacrol analogues was investigated by in-cage mosquito repellent bioassay. Results: The essential oil of the plant showed strong mosquito repellent activity with minimum effective dosage (MED) of 0.019 +/- 0.007 mg/cm(2), compared to reference standard N,N-diethyl-3-methylbenzamide (DEET) (0.031 +/- 0.014 mg/cm(2)). 26 compounds representing 97.8% of the essential oil were identified. Carvacrol, carvacrol methyl ether and a new fragrant compound, adenosmin A (1) were found to be repellent compounds by systematic bioassay-guided isolation, with MEDs in the range of 0.011-0.125 mg/cm(2). An investigation on SAR of carvacrol analogues led to the discover of three analogues with further lower MEDs (0.002-0.009 mg/cm(2)) than that of DEET, and other three compounds with similar MEDs (0.029-0.039 mg/cm(2)) to that of DEET. Carvacrol (LD50 of 24.8 ppm) was the best larvicide among tested repellent compounds. The essential oil and repellent compounds against seven mammalian cell lines revealed low or no cytotoxicity. Conclusions: Scientific evidences reported here validate the plant's traditional use as insect repellent and imply promising application of the essential oil and carvacrol analogues as natural mosquito repellents.
关键词	Adenosma buchneroides Ethnobotanical survey Mosquito repellent Carvacrol Structure-activity relationship
收录类别	SCI
语种	英语
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/64395
专题	资源植物与生物技术所级重点实验室
通讯作者	Du, Zhizhi
作者单位	1.Chinese Acad Sci, Key Lab Econ Plants & Biotechnol, Yunnan Key Lab Wild Plant Resources, Kunming Inst Bot, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Kunming City Ctr Dis Control & Prevent, Kunming 650034, Yunnan, Peoples R China
推荐引用方式 GB/T 7714	Ma, Yongpeng,Li, Mingxiang,Zhang, Hongxia,et al. Bioassay-guided isolation of active compounds from Adenosma buchneroides essential oil as mosquito repellent against Aedes albopictus[J]. JOURNAL OF ETHNOPHARMACOLOGY,2019,231:386-393.
APA	Ma, Yongpeng.,Li, Mingxiang.,Zhang, Hongxia.,Sun, Hongzheng.,Su, Honghai.,...&Du, Zhizhi.(2019).Bioassay-guided isolation of active compounds from Adenosma buchneroides essential oil as mosquito repellent against Aedes albopictus.JOURNAL OF ETHNOPHARMACOLOGY,231,386-393.
MLA	Ma, Yongpeng,et al."Bioassay-guided isolation of active compounds from Adenosma buchneroides essential oil as mosquito repellent against Aedes albopictus".JOURNAL OF ETHNOPHARMACOLOGY 231(2019):386-393.

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