Microbe-focused glycan array screening platform
Geissner, Andreas1,2,9; Reinhardt, Anika1,2,10; Rademacher, Christoph1,2; Johannssen, Timo1,2; Monteiro, Joao3,4; Lepenies, Bernd1,2,3,4; Thepaut, Michel5,6,7; Fieschi, Franck5,6,7; Mrazkova, Jana8; Wimmerova, Michaela8; Schuhmacher, Frank1,2; Goetze, Sebastian1,2,11; Gruenstein, Dan1,2; Guo, Xiaoqiang1,2,12; Hahm, Heung Sik1,2,13; Kandasamy, Jeyakumar1,14; Leonori, Daniele1,15; Martin, Christopher E.1,2,16; Parameswarappa, Sharavathi G.1,17; Pasari, Sandip1,18; Schlegel, Mark K.1,19; Tanaka, Hidenori1,20; Xiao, Guozhi1,21; Yang, You1,22; Pereira, Claney L.1,17; Anish, Chakkumkal1,23; Seeberger, Peter H.1,2
AbstractInteractions between glycans and glycan binding proteins are essential for numerous processes in all kingdoms of life. Glycan microarrays are an excellent tool to examine protein-glycan interactions. Here, we present a microbe-focused glycan microarray platform based on oligosaccharides obtained by chemical synthesis. Glycans were generated by combining different carbohydrate synthesis approaches including automated glycan assembly, solution-phase synthesis, and chemoenzymatic methods. The current library of more than 300 glycans is as diverse as the mammalian glycan array from the Consortium for Functional Glycomics and, due to its microbial focus, highly complementary. This glycan platform is essential for the characterization of various classes of glycan binding proteins. Applications of this glycan array platform are highlighted by the characterization of innate immune receptors and bacterial virulence factors as well as the analysis of human humoral immunity to pathogenic glycans.
Keywordglycan arrays microbial antigens immune receptors antiglycan antibodies bacterial lectins
Indexed BySCI
Document Type期刊论文
Corresponding AuthorSeeberger, Peter H.
Affiliation1.Max Planck Inst Colloids & Interfaces, Dept Biomol Syst, D-14424 Potsdam, Germany
2.Free Univ Berlin, Inst Chem & Biochem, D-14195 Berlin, Germany
3.Univ Vet Med Hannover, Immunol Unit, D-30559 Hannover, Germany
4.Univ Vet Med Hannover, Res Ctr Emerging Infect & Zoonoses, D-30559 Hannover, Germany
5.Univ Grenoble Alpes, Inst Biol Struct, F-38044 Grenoble, France
6.CNRS, Inst Biol Struct, F-38044 Grenoble, France
7.Commissariat Energie Atom & Energies Alternat, Inst Biol Struct, F-38044 Grenoble, France
8.Masaryk Univ, Cent European Inst Technol, Brno 62500, Czech Republic
9.Univ British Columbia, Dept Chem, Vancouver, BC V6T 1Z1, Canada
10.Kyoto Univ, Dept Life Sci Frontiers, Ctr iPS Cell Res & Applicat, Kyoto 6068507, Japan
11.Univ Koblenz Landau, Fac Nat & Environm Sci 7, D-76829 Landau, Germany
12.Clariant Innovat Ctr, D-65929 Frankfurt, Germany
13.Univ Virginia, Dept Chem, Charlottesville, VA 22904 USA
14.Indian Inst Technol, Dept Chem, Varanasi 221005, Uttar Pradesh, India
15.Univ Manchester, Sch Chem, Manchester M13 9PL, Lancs, England
16.Bachem AG, CH-4416 Bubendorf, Switzerland
17.Vaxxilon Deutschland GmbH, D-12489 Berlin, Germany
18.Indian Inst Sci Educ & Res, Pune 411008, Maharashtra, India
19.Alnylam Pharmaceut, Cambridge, MA 02142 USA
20.Kochi Univ, Ctr Sci Res, Oceanog Sect, Otsu 200, Nankoku, Kochi 7838503, Japan
21.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
22.East China Univ Sci & Technol, Sch Pharm, Shanghai Key Lab New Drug Design, Shanghai 200237, Peoples R China
23.Janssen Pharmaceut Johnson & Johnson, Bacterial Vaccines Discovery & Early Dev, NL-2333 CK Leiden, Netherlands
Recommended Citation
GB/T 7714
Geissner, Andreas,Reinhardt, Anika,Rademacher, Christoph,et al. Microbe-focused glycan array screening platform[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2019,116(6):1958-1967.
APA Geissner, Andreas.,Reinhardt, Anika.,Rademacher, Christoph.,Johannssen, Timo.,Monteiro, Joao.,...&Seeberger, Peter H..(2019).Microbe-focused glycan array screening platform.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,116(6),1958-1967.
MLA Geissner, Andreas,et al."Microbe-focused glycan array screening platform".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 116.6(2019):1958-1967.
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