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In vitro preservation and micropropagation of Oreocharis mileense (WT Wang) M. Moller & A. Weber (Gesneriaceae) through shoot organogenesis
Wang, Dandan1; Li, Xinhui2; Cheng, Zhiying3,4; Long, Chunlin3,4,5
2018-12-01
发表期刊IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT
ISSN1054-5476
卷号54期号:6页码:606-611
摘要Oreocharis mileense (W.T. Wang) M. Moller & A. Weber is endemic to China and was considered to be extinct because it had not been seen in the wild since the first collection in 1906. In 2006, the species was rediscovered in Shilin County, Yunnan Province. Oreocharis mileense was considered critically endangered for its narrow geographic range and extremely small population. An efficient method to preserve plant germplasm by in vitro culturing of O. mileense has not been reported. In this study, an orthogonal array with three factors (6-benzyladenine, BA; alpha-naphthaleneacetic acid, NAA; and sucrose), at four levels was performed, and shoot induction as well as shoot proliferation were recorded. The results were analyzed to determine the most significant components and the optimum combination for micropropagation of O. mileense. The results showed that: (1) organogenesis was easily induced by different combinations of plant-growth regulators and sucrose; (2) NAA and sucrose had the most significant effect on shoot induction and shoot multiplication, and (3) the optimum induction and proliferation media were 0.5 mg L-1 BA + 0.2 mg L-1 NAA + 30 g L-1 sucrose and 1 mg L-1 BA + 0.1 mg L-1 NAA + 30 g L-1 sucrose, respectively.
关键词Oreocharis mileense Rediscovery Critically endangered In vitro preservation Micropropagation
资助信息Ministry of Science and Technology of China; National Natural Science Foundation of China; Ministry of Education of China; Key Laboratory of Ethnomedicine (Minzu University of China) of the Ministry of Education of China
收录类别SCI
语种英语
资助项目Ministry of Science and Technology of China[2005DKA21006] ; Ministry of Science and Technology of China[2012FY110300] ; National Natural Science Foundation of China[31761143001] ; National Natural Science Foundation of China[31870316] ; National Natural Science Foundation of China[31070288] ; National Natural Science Foundation of China[31560063] ; Ministry of Education of China[yldxxk201819] ; Ministry of Education of China[2015MDTD16C] ; Ministry of Education of China[B08044] ; Key Laboratory of Ethnomedicine (Minzu University of China) of the Ministry of Education of China[KLEM-ZZ201806]
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/63282
专题中国科学院昆明植物研究所
通讯作者Long, Chunlin
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Yunnan, Peoples R China
2.Southwest Forestry Univ, Coll Ecol & Soil & Water Conservat, Kunming 650224, Yunnan, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Key Lab Econ Plants & Biotechnol, Kunming 650201, Yunnan, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resource, Kunming 650201, Yunnan, Peoples R China
5.Minzu Univ China, Coll Life & Environm Sci, Beijing 100081, Peoples R China
推荐引用方式
GB/T 7714
Wang, Dandan,Li, Xinhui,Cheng, Zhiying,et al. In vitro preservation and micropropagation of Oreocharis mileense (WT Wang) M. Moller & A. Weber (Gesneriaceae) through shoot organogenesis[J]. IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,2018,54(6):606-611.
APA Wang, Dandan,Li, Xinhui,Cheng, Zhiying,&Long, Chunlin.(2018).In vitro preservation and micropropagation of Oreocharis mileense (WT Wang) M. Moller & A. Weber (Gesneriaceae) through shoot organogenesis.IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT,54(6),606-611.
MLA Wang, Dandan,et al."In vitro preservation and micropropagation of Oreocharis mileense (WT Wang) M. Moller & A. Weber (Gesneriaceae) through shoot organogenesis".IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-PLANT 54.6(2018):606-611.
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