Transcriptome analysis reveals crucial genes involved in the biosynthesis of nervonic acid in woody Malania oleifera oilseeds
Yang, Tianquan1; Yu, Qian1,6; Xu, Wei3,4; Li, De-zhu1; Chen, Fu5; Liu, Aizhong2
2018-10-19
发表期刊BMC PLANT BIOLOGY
ISSN1471-2229
卷号18页码:13
摘要Background: Malania oleifera Chun et Lee (Olacaceae), an evergreen broad-leaved woody tree native to southwest China, is an important oilseed tree. Its seed oil has a high level of nervonic acid (cis-tetracos-15-enoic acid, over 60%), which is essential for human health. M. oleifera seed oil is a promising source of nervonic acid, but little is known about the physiological and molecular mechanisms underlying its biosynthesis. Results: In this study, we recorded oil accumulation at four stages of seed development. Using a high-throughput RNA-sequencing technique, we obtained 55,843 unigenes, of which 29,176 unigenes were functionally annotated. By comparison, 22,833 unigenes had a two-fold or greater expression at the fast oil accumulation stage than at the initial stage. Of these, 198 unigenes were identified as being functionally involved in diverse lipid metabolism processes (including de novo fatty acid synthesis, carbon chain elongation and modification, and triacylglycerol assembly). Key genes (encoding KCS, KCR, HCD and ECR), putatively responsible for nervonic acid biosynthesis, were isolated and their expression profiles during seed development were confirmed by quantitative real-time PCR analysis. Also, we isolated regulatory factors (such as WRI1, ABI3 and FUS3) that are putatively involved in the regulation of oil biosynthesis and seed development. Conclusion: Our results provide novel data on the physiological and molecular mechanisms of nervonic acid biosynthesis and oil accumulation in M. oleifera seeds, and will also serve as a starting point for biotechnological genetic engineering for the production of nervonic acid resources.
关键词Nervonic acids Malania oleifera Transcriptome Oilseed Gene expression Oil biosynthesis
资助信息National Natural Science Foundation of China; Yunnan Applied Basic Research Projects
DOI10.1186/s12870-018-1463-6
语种英语
资助项目National Natural Science Foundation of China[31700285] ; National Natural Science Foundation of China[31571709] ; Yunnan Applied Basic Research Projects[2018FB037]
WOS研究方向Plant Sciences
WOS类目Plant Sciences
WOS记录号WOS:000447772800001
出版者BMC
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/62564
专题资源植物与生物技术所级重点实验室
通讯作者Xu, Wei; Liu, Aizhong
作者单位1.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, 132 Lanhei Rd, Kunming 650204, Yunnan, Peoples R China
2.Southwest Forestry Univ, Minist Educ, Key Lab Forest Resources Conservat & Utilizat Sou, Kunming 650224, Yunnan, Peoples R China
3.Chinese Acad Sci, Kunming Inst Bot, Dept Econ Plants & Biotechnol, 132 Lanhei Rd, Kunming 650204, Yunnan, Peoples R China
4.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Wild Plant Resources, 132 Lanhei Rd, Kunming 650204, Yunnan, Peoples R China
5.Yunnan Acad Forestry, Camellia Inst, Kunming, Yunnan, Peoples R China
6.Univ Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714
Yang, Tianquan,Yu, Qian,Xu, Wei,et al. Transcriptome analysis reveals crucial genes involved in the biosynthesis of nervonic acid in woody Malania oleifera oilseeds[J]. BMC PLANT BIOLOGY,2018,18:13.
APA Yang, Tianquan,Yu, Qian,Xu, Wei,Li, De-zhu,Chen, Fu,&Liu, Aizhong.(2018).Transcriptome analysis reveals crucial genes involved in the biosynthesis of nervonic acid in woody Malania oleifera oilseeds.BMC PLANT BIOLOGY,18,13.
MLA Yang, Tianquan,et al."Transcriptome analysis reveals crucial genes involved in the biosynthesis of nervonic acid in woody Malania oleifera oilseeds".BMC PLANT BIOLOGY 18(2018):13.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Yang, Tianquan]的文章
[Yu, Qian]的文章
[Xu, Wei]的文章
百度学术
百度学术中相似的文章
[Yang, Tianquan]的文章
[Yu, Qian]的文章
[Xu, Wei]的文章
必应学术
必应学术中相似的文章
[Yang, Tianquan]的文章
[Yu, Qian]的文章
[Xu, Wei]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。