藏药“藏茵陈”的位点特异性PCR鉴别
李小娟 ; 马俊蓉 ; 薛春迎
2010
Source Publication昆明学院学报
ISSN1674-5639
Volume32Issue:6Pages:62-64
Abstract对与藏药"藏茵陈"药材质量、真伪密切相关的药材来源进行鉴别.首先考察、鉴定市售"藏茵陈"药材伪品原植物种,通过对这些植物进行分子序列分析,来比较正品原植物与伪品植物的分子序列碱基位点和分子片段的区别,从而筛选出可靠的分子标记来鉴别"藏茵陈"原植物川西獐牙菜Swertia mussotiiFranch.的真伪.根据"藏茵陈"原植物特异性的分子片段,设计专用于鉴别川西獐牙菜的PCR引物,用该引物在不同条件下扩增正品及伪品"藏茵陈"模板DNA,建立只有正品川西獐牙菜具有阳性扩增的PCR鉴别条件.从而建立一种简便、适用的"藏茵陈"川西獐牙菜的DNA分子鉴别方法.
Keyword藏药 藏茵陈 川西獐牙菜 分子鉴定
Indexed Bycscd
Language中文
Citation statistics
Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/6235
Collection中国科学院东亚植物多样性与生物地理学重点实验室
Recommended Citation
GB/T 7714
李小娟,马俊蓉,薛春迎. 藏药“藏茵陈”的位点特异性PCR鉴别[J]. 昆明学院学报,2010,32(6):62-64.
APA 李小娟,马俊蓉,&薛春迎.(2010).藏药“藏茵陈”的位点特异性PCR鉴别.昆明学院学报,32(6),62-64.
MLA 李小娟,et al."藏药“藏茵陈”的位点特异性PCR鉴别".昆明学院学报 32.6(2010):62-64.
Files in This Item: Download All
File Name/Size DocType Version Access License
20111203087.pdf(801KB) 开放获取--View Download
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[李小娟]'s Articles
[马俊蓉]'s Articles
[薛春迎]'s Articles
Baidu academic
Similar articles in Baidu academic
[李小娟]'s Articles
[马俊蓉]'s Articles
[薛春迎]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[李小娟]'s Articles
[马俊蓉]'s Articles
[薛春迎]'s Articles
Terms of Use
No data!
Social Bookmark/Share
File name: 20111203087.pdf
Format: Adobe PDF
This file does not support browsing at this time
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.