KIB OpenIR
Lipid profiles reveal different responses to brown planthopper infestation for pest susceptible and resistant rice plants
Zhang, Jiajiao1; Li, Yi1; Guo, Jianping1; Du, Bo1; He, Guangcun1; Zhang, Yingjun2; Chen, Rongzhi1; Li, Jiaru1
2018-09-01
发表期刊METABOLOMICS
ISSN1573-3882
卷号14期号:9页码:13
摘要IntroductionBrown planthopper (BPH) is the most destructive insect pest for rice, causing major reductions in rice yield and large economic losses. More than 31 BPH-resistance genes have been located, and several of them have been isolated. Nevertheless, the metabolic mechanism related to BPH-resistance genes remain uncharacterized.ObjectivesTo elucidate the resistance mechanism of the BPH-resistance gene Bph6 at the metabolic level, a Bph6-transgenic line R6 (BPH-resistant) and the wild-type Nipponbare (BPH-susceptible) were used to investigate their lipid profiles under control and BPH treatments.MethodsIn conjunction with multivariate statistical analysis and quantitative real-time PCR, BPH-induced lipid changes in leaf blade and leaf sheath were investigated by GC-MS-based lipidomics.ResultsForty-five lipids were identified in leaf sheath extracts. Leaf sheath lipidomics analysis results show that BPH infestation induces significant differences in the lipid profiles of Nipponbare and R6. The levels of hexadecanoic acid, methyl ester, linoleic acid, methyl ester, linolenic acid, methyl ester, glycidyl palmitate, eicosanoic acid, methyl ester, docosanoic acid, methyl ester, beta-monolinolein, campesterol, beta-sitosterol, cycloartenol, phytol and phytyl acetate had undergone enormous changes after BPH feeding. These results illustrate that BPH feeding enhances sterol biosynthetic pathway in Nipponbare plants, and strengthens wax biosynthesis and phytol metabolism in R6 plants. The results of quantitative real-time PCR of 5 relevant genes were consistent with the changes in metabolic level. Forty-five lipids were identified in the leaf blade extracts. BPH infestation induces distinct changes in the lipid profiles of the leaf blade samples of Nipponbare and R6. Although the lipid changes in Nipponbare are more drastic, the changes within the two varieties are similar. Lipid profiles in leaf sheath brought out significant differences than in leaf blade within Nipponbare and R6. We propose that Bph6 mainly affects the levels of lipids in leaf sheath, and mediates resistance by deploying metabolic re-programming during BPH feeding.ConclusionThe results indicate that wax biosynthesis, sterol biosynthetic pathway and phytol metabolism play vital roles in rice response to BPH infestation. This finding demonstrated that the combination of lipidomics and quantitative real-time PCR is an effective approach to elucidating the interactions between brown planthopper and rice mediated by resistance genes.
关键词Rice plants Brown planthopper Lipidomics GC-MS Quantitative real-time PCR
资助信息National Program on Research and Development of Transgenic Plants; National Key Research and Development Program
语种英语
资助项目National Program on Research and Development of Transgenic Plants[2016ZX08009-003-001] ; National Key Research and Development Program[2016YFD0100600] ; National Key Research and Development Program[2016YFD0100900]
引用统计
文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/62236
专题中国科学院昆明植物研究所
通讯作者Zhang, Yingjun; Chen, Rongzhi; Li, Jiaru
作者单位1.Wuhan Univ, Coll Life Sci, State Key Lab Hybrid Rice, Wuhan 430072, Hubei, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China
推荐引用方式
GB/T 7714
Zhang, Jiajiao,Li, Yi,Guo, Jianping,et al. Lipid profiles reveal different responses to brown planthopper infestation for pest susceptible and resistant rice plants[J]. METABOLOMICS,2018,14(9):13.
APA Zhang, Jiajiao.,Li, Yi.,Guo, Jianping.,Du, Bo.,He, Guangcun.,...&Li, Jiaru.(2018).Lipid profiles reveal different responses to brown planthopper infestation for pest susceptible and resistant rice plants.METABOLOMICS,14(9),13.
MLA Zhang, Jiajiao,et al."Lipid profiles reveal different responses to brown planthopper infestation for pest susceptible and resistant rice plants".METABOLOMICS 14.9(2018):13.
条目包含的文件
条目无相关文件。
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[Zhang, Jiajiao]的文章
[Li, Yi]的文章
[Guo, Jianping]的文章
百度学术
百度学术中相似的文章
[Zhang, Jiajiao]的文章
[Li, Yi]的文章
[Guo, Jianping]的文章
必应学术
必应学术中相似的文章
[Zhang, Jiajiao]的文章
[Li, Yi]的文章
[Guo, Jianping]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。