Anti-hepatitis B virus effects of the traditional Chinese herb Arteinisia capillaris and its active enynes

doi:10.1016/j.jep.2018.06.005

KIB OpenIR

	Anti-hepatitis B virus effects of the traditional Chinese herb Arteinisia capillaris and its active enynes
	Geng, Chang-An1 ; Yang, Tong-Hua 1; Huang, Xiao-Yan 1; Yang, Jing 1; Ma, Yun-Bao 1; Li, Tian-Ze 1; Zhang, Xue-Mei 1; Chen, Ji-Jun1,2
	2018-10-05
发表期刊	JOURNAL OF ETHNOPHARMACOLOGY
ISSN	0378-8741
卷号	224 页码:283-289
摘要	Ethnopharmacological relevance: Artemisia capillaris (Yin-Chen) is a famous traditional Chinese medicine (TCM) for treating acute and chronic hepatitis in China. Enynes are one type of characteristic constituents in this herb, while their anti-hepatitis B virus (anti-HBV) properties have not been systemically investigated. Aim of the study: This study is to reveal the active part of A. capillaris, and systemically investigate the enynes and their anti-HBV activity. Materials and methods: The total extract and each fraction of A. capillaris were assayed for the anti-HBV activity to reveal the active part. Bioassay-guided fractionation using various chromatographic techniques yielded the enynes, whose structures were elucidated by spectroscopic analyses and ECD calculations. The anti-HBV properties inhibiting HBsAg and HBeAg secretions and HBV DNA replication were evaluated on HepG 2.2.15 cell line in vitro. Results: ACT-2 and ACT-3 was revealed to be the respective active and toxic part of A. capillaris. Twelve enynes (1-12) involving four new ones (1-4) and two unusual enyne analogs (13-14) were isolated from the active part (ACT-2). All the isolates were assayed for their anti-HBV activity, and the preliminary structure-activity relationships were summarized based on the structural features. In particular, compound 4 could significantly inhibit the secretions of HBsAg and HBeAg, and HBV DNA replication with IC50 values of 197.2 (SI > 5.1), 48.7 (SI > 20.5) and 9.8 (SI > 102) mu M. Conclusions: Enynes are responsible for the anti-HBV effects of A. capillaris. Hydroxyl and glycosyl groups are preferable for maintaining activity. This is the first time to systematically investigate the anti-HBV activity of enynes in A. capillaris, which provides valuable information for understanding the ethnopharmacological application of Yin-Chen.
关键词	Artemisia capillaris Enynes Anti-HBV activity Structure-activity relationships
DOI	10.1016/j.jep.2018.06.005
语种	英语
WOS记录号	WOS:000442333900026
引用统计	被引频次：32[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.kib.ac.cn/handle/151853/62140
专题	中国科学院昆明植物研究所植物化学与西部植物资源持续利用国家重点实验室
通讯作者	Chen, Ji-Jun
作者单位	1.Chinese Acad Sci, Kunming Inst Bot, Yunnan Key Lab Nat Med Chem, State Key Lab Phytochem & Plant Resources West Ch, Kunming 650201, Yunnan, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式 GB/T 7714	Geng, Chang-An,Yang, Tong-Hua,Huang, Xiao-Yan,et al. Anti-hepatitis B virus effects of the traditional Chinese herb Arteinisia capillaris and its active enynes[J]. JOURNAL OF ETHNOPHARMACOLOGY,2018,224:283-289.
APA	Geng, Chang-An.,Yang, Tong-Hua.,Huang, Xiao-Yan.,Yang, Jing.,Ma, Yun-Bao.,...&Chen, Ji-Jun.(2018).Anti-hepatitis B virus effects of the traditional Chinese herb Arteinisia capillaris and its active enynes.JOURNAL OF ETHNOPHARMACOLOGY,224,283-289.
MLA	Geng, Chang-An,et al."Anti-hepatitis B virus effects of the traditional Chinese herb Arteinisia capillaris and its active enynes".JOURNAL OF ETHNOPHARMACOLOGY 224(2018):283-289.