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Integrating a comprehensive DNA barcode reference library with a global map of yews (Taxus L.) for forensic identification
Liu, Jie1,2; Milne, Richard I.3; Moller, Michael4; Zhu, Guang-Fu1,2,5; Ye, Lin-Jiang1,2,5; Luo, Ya-Huang1; Yang, Jun-Bo2; Wambulwa, Moses C.6; Wang, Chun-Neng7; Li, De-Zhu2,5; Gao, Lian-Ming1
2018-09-01
Source PublicationMOLECULAR ECOLOGY RESOURCES
ISSN1755-098X
Volume18Issue:5Pages:1115-1131
Abstract

Rapid and accurate identification of endangered species is a critical component of biosurveillance and conservation management, and potentially policing illegal trades. However, this is often not possible using traditional taxonomy, especially where only small or preprocessed parts of plants are available. Reliable identification can be achieved via a comprehensive DNA barcode reference library, accompanied by precise distribution data. However, these require extensive sampling at spatial and taxonomic scales, which has rarely been achieved for cosmopolitan taxa. Here, we construct a comprehensive DNA barcode reference library and generate distribution maps using species distribution modelling (SDM), for all 15 Taxus species worldwide. We find that trnL-trnF is the ideal barcode for Taxus: It can distinguish all Taxus species and in combination with ITS identify hybrids. Among five analysis methods tested, NJ was the most effective. Among 4,151 individuals screened for trnL-trnF, 73 haplotypes were detected, all species-specific and some population private. Taxonomical, geographical and genetic dimensions of sampling strategy were all found to affect the comprehensiveness of the resulting DNA barcode library. Maps from SDM showed that most species had allopatric distributions, except T. mairei in the Sino-Himalayan region. Using the barcode library and distribution map data, two unknown forensic samples were identified to species (and in one case, population) level and another was determined as a putative interspecific hybrid. This integrated species identification system for Taxus can be used for biosurveillance, conservation management and to monitor and prosecute illegal trade. Similar identification systems are recommended for other IUCN- and CITES-listed taxa.

KeywordComprehensive Sampling Dna Barcoding Forensic Identification Geographical Origin Sampling Strategy Species Distribution Modelling
DOI10.1111/1755-0998.12903
Language英语
WOS Research AreaBiochemistry & Molecular Biology ; Environmental Sciences & Ecology ; Evolutionary Biology
WOS SubjectBiochemistry & Molecular Biology ; Ecology ; Evolutionary Biology
WOS IDWOS:000441753000016
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Document Type期刊论文
Identifierhttp://ir.kib.ac.cn/handle/151853/62078
Collection中国科学院昆明植物研究所
Corresponding AuthorLi, De-Zhu; Gao, Lian-Ming
Affiliation1.Chinese Acad Sci, Kunming Inst Bot, Key Lab Plant Divers & Biogeog East Asia, Kunming, Yunnan, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming, Yunnan, Peoples R China
3.Univ Edinburgh, Inst Mol Plant Sci, Sch Biol Sci, Edinburgh, Midlothian, Scotland
4.Royal Bot Garden Edinburgh, Edinburgh, Midlothian, Scotland
5.Univ Chinese Acad Sci, Coll Life Sci, Kunming, Yunnan, Peoples R China
6.South Eastern Kenya Univ, Dept Biochem, Kitui, Kenya
7.Natl Taiwan Univ, Inst Ecol & Evolutionary Biol, Dept Life Sci, Taipei, Taiwan
Recommended Citation
GB/T 7714
Liu, Jie,Milne, Richard I.,Moller, Michael,et al. Integrating a comprehensive DNA barcode reference library with a global map of yews (Taxus L.) for forensic identification[J]. MOLECULAR ECOLOGY RESOURCES,2018,18(5):1115-1131.
APA Liu, Jie.,Milne, Richard I..,Moller, Michael.,Zhu, Guang-Fu.,Ye, Lin-Jiang.,...&Gao, Lian-Ming.(2018).Integrating a comprehensive DNA barcode reference library with a global map of yews (Taxus L.) for forensic identification.MOLECULAR ECOLOGY RESOURCES,18(5),1115-1131.
MLA Liu, Jie,et al."Integrating a comprehensive DNA barcode reference library with a global map of yews (Taxus L.) for forensic identification".MOLECULAR ECOLOGY RESOURCES 18.5(2018):1115-1131.
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