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Genetic Evaluation of Natural Populations of the Endangered Conifer Thuja koraiensis Using Microsatellite Markers by Restriction-Associated DNA Sequencing
Hou, Lu1; Cui, Yanhong1; Li, Xiang1; Chen, Wu2; Zhang, Zhiyong3; Pang, Xiaoming1; Li, Yingyue1
2018-04-01
发表期刊GENES
ISSN2073-4425
卷号9期号:4
摘要Thuja koraiensis Nakai is an endangered conifer of high economic and ecological value in Jilin Province, China. However, studies on its population structure and conservation genetics have been limited by the lack of genomic data. Here, 37,761 microsatellites (simple sequence repeat, SSR) were detected based on 875,792 de novo-assembled contigs using a restriction-associated DNA (RAD) approach. Among these SSRs, 300 were randomly selected to test for polymorphisms and 96 obtained loci were able to amplify a fragment of expected size. Twelve polymorphic SSR markers were developed to analyze the genetic diversity and population structure of three natural populations. High genetic diversity (mean N-A = 5.481, H-E = 0.548) and moderate population differentiation (pairwise F-st = 0.048-0.078, N-m = 2.940-4.958) were found in this species. Molecular variance analysis suggested that most of the variation (83%) existed within populations. Combining the results of STRUCTURE, principal coordinate, and neighbor-joining analysis, the 232 individuals were divided into three genetic clusters that generally correlated with their geographical distributions. Finally, appropriate conservation strategies were proposed to protect this species. This study provides genetic information for the natural resource conservation and utilization of T. koraiensis and will facilitate further studies of the evolution and phylogeography of the species.
关键词Thuja Koraiensis Nakai Restriction-associated Dna (Rad) Sequencing Simple Sequence Repeat (Ssr) Markers Population Genetics Conservation Genetics
语种英语
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文献类型期刊论文
条目标识符http://ir.kib.ac.cn/handle/151853/61382
专题中国西南野生生物种质资源库
作者单位1.Beijing Forestry Univ, Coll Biol Sci & Technol, Natl Engn Lab Tree Breeding, Beijing 100083, Peoples R China
2.Chinese Acad Sci, Kunming Inst Bot, Germplasm Bank Wild Species, Kunming 650201, Yunnan, Peoples R China
3.Beijing Forestry Univ, Beijing Key Lab Ornamental Plants Germplasm Innov, Natl Engn Res Ctr Floriculture,Minist Educ,Sch La, Beijing Lab Urban & Rural Ecol Environm,Key Lab G, Beijing 100083, Peoples R China
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GB/T 7714
Hou, Lu,Cui, Yanhong,Li, Xiang,et al. Genetic Evaluation of Natural Populations of the Endangered Conifer Thuja koraiensis Using Microsatellite Markers by Restriction-Associated DNA Sequencing[J]. GENES,2018,9(4).
APA Hou, Lu.,Cui, Yanhong.,Li, Xiang.,Chen, Wu.,Zhang, Zhiyong.,...&Li, Yingyue.(2018).Genetic Evaluation of Natural Populations of the Endangered Conifer Thuja koraiensis Using Microsatellite Markers by Restriction-Associated DNA Sequencing.GENES,9(4).
MLA Hou, Lu,et al."Genetic Evaluation of Natural Populations of the Endangered Conifer Thuja koraiensis Using Microsatellite Markers by Restriction-Associated DNA Sequencing".GENES 9.4(2018).
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